Simple Plex Non-Human Primate GFAP 2nd Gen Cartridge

R&D Systems | Catalog # SPCKB-NP-016651

Simple Plex Non-Human Primate GFAP assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer. Sample type indicated by PS (Plasma/Serum).
R&D Systems

Customize This Cartridge

Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.

Customize This Cartridge

Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (25 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL), Cerebrospinal Fluid (25 µL)

Sensitivity

1.08 pg/mL

Assay Range

2.52-9600 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid)

Simple Plex Non-Human Primate GFAP 2nd Gen Cartridge Summary

Simple Plex assay is for the detection of non-human primate GFAP in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), and cerebrospinal fluid (CSF) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all GFAP Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge with 3 Glass Nano Reactors (GNR)

Species

Non-human Primate

Specificity

Natural and recombinant GFAP.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one GFAP assay.

Inter-Assay Precision (Precision between assays) Replicates of each GFAP control were tested in multiple assays performed by at least three technicians using two lots of reagents for non-human primate GFAP.

Cell Culture Supernates, Cerebrospinal Fluid, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 13 13
Mean (pg/mL) 86.5 3501 84.3 3445
Standard Deviation 3.56 134 7.93 269
CV% 4.1 3.8 9.4 7.8

Recovery for Simple Plex Non-Human Primate GFAP 2nd Gen Cartridge

Recovery for the non-human primate GFAP assays was evaluated at three different spiked concentrations of GFAP protein.

Sample Type Average % Recovery Range %
Cell Culture Media (n=2) 114 110-119
EDTA Plasma (n=4) 96 90-102
Heparin Plasma (n=6) 90 79-110
Serum (n=5) 93 78-101

Linearity

Samples containing and/or spiked with high concentrations of GFAP were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Simple Plex Non-Human Primate GFAP 2nd Generation Assay Linearity

Scientific Data Images for Simple Plex Non-Human Primate GFAP 2nd Gen Cartridge

Simple Plex Non-Human Primate GFAP 2nd Generation Assay Standard Curve

Simple Plex Non-Human Primate GFAP 2nd Generation Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Non-Human Primate GFAP Assay. Assay range is 2.52-9600 pg/mL.
Simple Plex Non-Human Primate GFAP 2nd Generation Assay Endogenous Levels

Simple Plex Non-Human Primate GFAP 2nd Generation Assay Endogenous Levels

Functional LOD and functional LLOQ are calculated as the LOD and LLOQ of the assay multiplied by the minimum required dilution (MRD) for the sample type.

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: GFAP

Glial Fibrillary Acidic Protein (GFAP) is a type III intermediate filament protein. GFAP is the predominant component of astrocyte intermediate filaments in the central nervous system and is often used as an astrocytic marker. It has also been detected in the glial cells of the enteric nervous system and some Schwann cells in the peripheral nervous system.

Long Name

Glial Fibrillary Acidic Protein

Alternate Names

ALXDRD, FLJ45472, GFAP, GFAP astrocytes, glial fibrillary acidic protein

Entrez Gene IDs

2670 (Human); 14580 (Mouse); 24387 (Rat)

Gene Symbol

GFAP

Additional GFAP Products

Product Documents for Simple Plex Non-Human Primate GFAP 2nd Gen Cartridge

Loading product document information...

Loading product document information...

Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex Non-Human Primate GFAP 2nd Gen Cartridge

For research use only

Citations for Simple Plex Non-Human Primate GFAP 2nd Gen Cartridge

View all citations for the Simple Plex platform.

Please visit our Instrument Citation Database to search our collection of scientific articles that feature our instruments.

Customer Reviews for Simple Plex Non-Human Primate GFAP 2nd Gen Cartridge

There are currently no reviews for this product. Be the first to review Simple Plex Non-Human Primate GFAP 2nd Gen Cartridge and earn rewards!

Have you used Simple Plex Non-Human Primate GFAP 2nd Gen Cartridge?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 for a review with an image

$10/€7/£6/$10CAN/¥1110 for a review without an image

Submit a review
Amazon Gift Card