Simple Plex Rat NF-L Cartridge

R&D Systems | Catalog # SPCKB-RP-003406

Simple Plex Rat NF-L assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)

Sensitivity

1.09 pg/mL

Assay Range

2.7-10290 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Rat NF-L Cartridge Summary

Simple Plex assay is for the detection of rat Neurofilament Light (NF-L) in serum and plasma (EDTA/Heparin) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all NF-L Simple Plex »

Compatible Instruments

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Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
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Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Rat

Specificity

Natural and recombinant NF-L.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one NF-L assay.

Inter-Assay Precision (Precision between assays) Replicates of each NF-L control were tested in multiple assays performed by at least three technicians using two lots of reagents for rat NF-L.

EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 2 1
n 16 16 48 48
Mean (pg/mL) 76.6 80.5 3476 3309
Standard Deviation 3.64 8.35 267 154
CV% 4.8 10.4 7.7 4.7

Recovery for Simple Plex Rat NF-L Cartridge

Recovery for the rat NF-L assays was evaluated at three different spiked concentrations of NF-L protein.

Sample Type Average % Recovery Range %
EDTA Plasma (n=3) 81 79-82
Heparin Plasma (n=4) 86 81-94
Serum (n=4) 87 79-92

Linearity

Samples containing and/or spiked with high concentrations of NF-L were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Simple Plex Rat NF-L Assay Linearity

Scientific Data Images for Simple Plex Rat NF-L Cartridge

Simple Plex Rat NF-L Assay Standard Curve

Simple Plex Rat NF-L Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Rat NF-L Assay. Assay range is 2.70-10,290 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: NF-L

Neurofilaments are intermediate filaments that are major components of the neuronal cytoskeleton. Neurofilaments are composed of three subunits: the heavy subunit (NF-H), medium subunit (NF-M), and light subunit (NF-L). Neurofilaments are trimers that always contain the 68 kDa NF-L and variably contain 125 kDa NF-M and 200 kDa NF-H. The human neurofilament heavy chain is a phosphorylated cytoskeletal intermediate filament protein that is expressed in neurons. The neurofilament medium subunit Human NF-M is a phosphorylated glycoprotein that belongs to the type IV intermediate filament family. NF-L is a 68 kDa light chain cytoskeletal intermediate filament protein that associates with the 125 kDa NF-M and the 200 kDa NF-H to form neurofilaments.

All three neurofilament subunits share a common structure, with globular head, alpha-helical rod, and carboxy terminal side-arm domains. The subunits assemble to form 10 nm neurofilaments. Accumulation of neurofilaments is seen in several human diseases including amyotrophic lateral sclerosis (ALS), Parkinsons disease, Alzheimers disease, and diabetic neuropathy.

Long Name

Neurofilament Protein, Light Chain

Alternate Names

CMT2E, NEFL, NF68, NFL

Entrez Gene IDs

4747 (Human)

Gene Symbol

NEFL

Additional NF-L Products

Product Documents for Simple Plex Rat NF-L Cartridge

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Certificate of Analysis

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Product Specific Notices for Simple Plex Rat NF-L Cartridge

For research use only

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