Simple Plex Rat TIM-1/KIM-1/HAVCR Cartridge

R&D Systems | Catalog # SPCKB-RP-000548

Simple Plex Rat TIM-1/KIM-1/HAVCR assay kit for use on Ella instrument. Contains cartridge, sample diluent SD09, and wash buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Rat Serum (10 µL), Rat EDTA Plasma (10 µL), Rat Urine (10 µL)

Sensitivity

2.89 pg/mL

Assay Range

7.06-10770 pg/mL (Cell Culture Supernates, Rat Serum, Rat EDTA Plasma, Rat Urine)

Simple Plex Rat TIM-1/KIM-1/HAVCR Cartridge Summary

Simple Plex assay is for the detection of rat T Cell Immunoglobulin Mucin (TIM-1) in serum, EDTA plasma, and urine in Sample Diluent SD09.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all TIM-1/KIM-1/HAVCR Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
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Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Rat

Specificity

Natural and recombinant rat TIM-1.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one TIM-1 assay.

Inter-Assay Precision (Precision between assays) Replicates of each TIM-1 control were tested in multiple assays performed by at least three technicians using two lots of reagents for rat TIM-1.

Cell Culture Supernates, Rat EDTA Plasma, Rat Serum, Rat Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 21 19
Mean (pg/mL) 53.5 2476 50.9 2593
Standard Deviation 2.80 176 4.57 214
CV% 5.2 7.1 9.0 8.3

Recovery for Simple Plex Rat TIM-1/KIM-1/HAVCR Cartridge

Recovery for the rat TIM-1 assays was evaluated at three different spiked concentrations of TIM-1 protein.

Sample Type Average % Recovery Range %
Rat EDTA Plasma (n=4) 102 94-113
Rat Serum (n=4) 103 94-111
Rat Urine (n=4) 110 103-115

Linearity

Samples containing and/or spiked with high concentrations of rat TIM-1 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Rat TIM-1/KIM-1/HAVCR Simple Plex Assay Linearity

Scientific Data Images for Simple Plex Rat TIM-1/KIM-1/HAVCR Cartridge

Simple Plex Rat TIM-1 Assay Standard Curve

Simple Plex Rat TIM-1 Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Rat TIM-1 Assay. Assay range is 7.06-10,770 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TIM-1/KIM-1/HAVCR

TIM-1 (T cell immunoglobulin and mucin domain 1), also known as KIM-1 and HAVcr1, is expressed on many immune cell types and epithelial cells. It binds to phosphatidylserine (PS), LMIR5/CD300b, TIM-1 (homophilic), TIM-4, IgA, and the glycoproteins of a number of enveloped viruses. Its interaction with PS enables TIM-1 to mediate the phagocytosis and clearance of apoptotic cells. TIM-1 signaling costimulates T cell activation and enhances Th2 cytokine production. TIM-1 serves as a cellular entry receptor for hepatitis A virus, Ebolavirus and Marburgvirus. Polymorphisms are associated with susceptibility to atopy, autoimmunity, and severe hepatitis A virus infection in humans. A soluble form of TIM-1 is elevated in the urine during nephropathy.

Long Name

T Cell Immunoglobulin Mucin-1

Alternate Names

CD365, HAVCR1, KIM-1, TIM1

Entrez Gene IDs

26762 (Human); 171283 (Mouse); 286934 (Rat); 102141332 (Cynomolgus Monkey)

Gene Symbol

HAVCR1

Additional TIM-1/KIM-1/HAVCR Products

Product Documents for Simple Plex Rat TIM-1/KIM-1/HAVCR Cartridge

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Certificate of Analysis

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Product Specific Notices for Simple Plex Rat TIM-1/KIM-1/HAVCR Cartridge

For research use only

Citations for Simple Plex Rat TIM-1/KIM-1/HAVCR Cartridge

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