Simple Plex Ultra-Sensitive Human GFAP Assay

R&D Systems | Catalog # SPCKB-PS-015539

Simple Plex Ultra-Sensitive Human GFAP assay kit for use on Ella instrument. Contains cartridge, Sample Diluent SD13, and Wash Buffer.
R&D Systems

Key Product Details

Assay Length

2 hours 50 minutes

Sample Type & Volume Required Per Well*

Serum (40 µL), EDTA Plasma (40 µL), Heparin Plasma (40 µL), Cerebrospinal Fluid (40 µL)

Sensitivity

0.19 pg/mL

Assay Range

0.62-5908 pg/mL (Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid)

Simple Plex Ultra-Sensitive Human GFAP Assay Summary

Simple Plex Ultra-Sensitive Assay is for the detection of human Glial Fibrillary Acidic Protein (GFAP) in serum, plasma (EDTA/Heparin), and cerebrospinal fluid (CSF) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all GFAP Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

icrofluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human GFAP.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one GFAP assay.

Inter-Assay Precision (Precision between assays) Replicates of each GFAP control were tested in multiple assays performed by at least three technicians using two lots of reagents for human GFAP.

Cerebrospinal Fluid, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 72 73
Mean (pg/mL) 46.1 2063 45.8 1923
Standard Deviation 2.263 94.8 5.40 171
CV% 4.9 4.6 11.8 8.9

Recovery for Simple Plex Ultra-Sensitive Human GFAP Assay

Recovery for the human GFAP assays was evaluated at three different spiked concentrations of GFAP protein.

Sample Type Average % Recovery Range %
EDTA Plasma (n=5) 82 68-98
Heparin Plasma (n=4) 99 73-112
Serum (n=5) 79 66-98

Linearity

Samples containing and/or spiked with high concentrations of GFAP were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Simple Plex Ultra-Sensitive Human GFAP Assay Linearity

Scientific Data Images for Simple Plex Ultra-Sensitive Human GFAP Assay

Simple Plex Ultra-Sensitive Human GFAP Assay Standard Curve

Simple Plex Ultra-Sensitive Human GFAP Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Ultra-Sensitive Human GFAP Assay. Assay range is 0.62-5908 pg/mL.
Simple Plex Ultra-Sensitive Human GFAP Assay Endogenous Levels

Simple Plex Ultra-Sensitive Human GFAP Assay Endogenous Levels

Functional LOD and functional LLOQ are calculated as the LOD and LLOQ of the assay multiplied by the minimum required dilution (MRD) for the sample type.

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: GFAP

Glial Fibrillary Acidic Protein (GFAP) is a type III intermediate filament protein. GFAP is the predominant component of astrocyte intermediate filaments in the central nervous system and is often used as an astrocytic marker. It has also been detected in the glial cells of the enteric nervous system and some Schwann cells in the peripheral nervous system.

Long Name

Glial Fibrillary Acidic Protein

Alternate Names

ALXDRD, FLJ45472, GFAP, GFAP astrocytes, glial fibrillary acidic protein

Entrez Gene IDs

2670 (Human); 14580 (Mouse); 24387 (Rat)

Gene Symbol

GFAP

Additional GFAP Products

Product Documents for Simple Plex Ultra-Sensitive Human GFAP Assay

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Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex Ultra-Sensitive Human GFAP Assay

For research use only

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