Simple Plex Ultra-Sensitive Human NF-L Assay

R&D Systems | Catalog # SPCKB-PS-015538

Simple Plex Ultra-Sensitve Human NF-L assay kit for use on Ella instrument. Contains cartridge, Sample Diluent SD13, and Wash Buffer.
R&D Systems

Key Product Details

Assay Length

2 hours 50 minutes

Sample Type & Volume Required Per Well*

Serum (40 µL), EDTA Plasma (40 µL), Heparin Plasma (40 µL), Cerebrospinal Fluid (40 µL)

Sensitivity

0.37 pg/mL

Assay Range

1.99-7600 pg/mL (Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid)

Simple Plex Ultra-Sensitive Human NF-L Assay Summary

Simple Plex Ultra-Sensitive Assay is for the detection of human Neurofilament Light (NF-L) in serum, plasma (EDTA/Heparin), and cerebrospinal fluid (CSF) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all NF-L Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human NF-L.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one NF-L assay.

Inter-Assay Precision (Precision between assays) Replicates of each NF-L control were tested in multiple assays performed by at least three technicians using two lots of reagents for human NF-L.

Cerebrospinal Fluid, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 90 86
Mean (pg/mL) 66.2 3031 77.1 3280
Standard Deviation 1.21 78.3 8.44 205
CV% 1.8 2.6 10.9 6.3

Recovery for Simple Plex Ultra-Sensitive Human NF-L Assay

Recovery for the human NF-L assays was evaluated at three different spiked concentrations of NF-L protein.

Sample Type Average % Recovery Range %
EDTA Plasma (n=5) 86 78-97
Heparin Plasma (n=4) 93 88-99
Serum (n=4) 83 67-101

Linearity

Samples containing and/or spiked with high concentrations of NF-L were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Simple Plex Ultra-Sensitive Human NF-L Assay Linearity

Scientific Data Images for Simple Plex Ultra-Sensitive Human NF-L Assay

Simple Plex Ultra-Sensitive Human NF-L Assay Standard Curve

Simple Plex Ultra-Sensitive Human NF-L Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Ultra-Sensitive Human NF-L Assay. Assay range is 1.99-7600 pg/mL.
Simple Plex Ultra-Sensitive Human NF-L Assay Endogenous Levels

Simple Plex Ultra-Sensitive Human NF-L Assay Endogenous Levels

Functional LOD and functional LLOQ are calculated as the LOD and LLOQ of the assay multiplied by the minimum required dilution (MRD) for the sample type.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: NF-L

Neurofilaments are intermediate filaments that are major components of the neuronal cytoskeleton. Neurofilaments are composed of three subunits: the heavy subunit (NF-H), medium subunit (NF-M), and light subunit (NF-L). Neurofilaments are trimers that always contain the 68 kDa NF-L and variably contain 125 kDa NF-M and 200 kDa NF-H. The human neurofilament heavy chain is a phosphorylated cytoskeletal intermediate filament protein that is expressed in neurons. The neurofilament medium subunit Human NF-M is a phosphorylated glycoprotein that belongs to the type IV intermediate filament family. NF-L is a 68 kDa light chain cytoskeletal intermediate filament protein that associates with the 125 kDa NF-M and the 200 kDa NF-H to form neurofilaments.

All three neurofilament subunits share a common structure, with globular head, alpha-helical rod, and carboxy terminal side-arm domains. The subunits assemble to form 10 nm neurofilaments. Accumulation of neurofilaments is seen in several human diseases including amyotrophic lateral sclerosis (ALS), Parkinsons disease, Alzheimers disease, and diabetic neuropathy.

Long Name

Neurofilament Protein, Light Chain

Alternate Names

CMT2E, NEFL, NF68, NFL

Entrez Gene IDs

4747 (Human)

Gene Symbol

NEFL

Additional NF-L Products

Product Documents for Simple Plex Ultra-Sensitive Human NF-L Assay

Loading product document information...

Loading product document information...

Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex Ultra-Sensitive Human NF-L Assay

For research use only

Citations for Simple Plex Ultra-Sensitive Human NF-L Assay

View all citations for the Simple Plex platform.

Please visit our Instrument Citation Database to search our collection of scientific articles that feature our instruments.

Customer Reviews for Simple Plex Ultra-Sensitive Human NF-L Assay

There are currently no reviews for this product. Be the first to review Simple Plex Ultra-Sensitive Human NF-L Assay and earn rewards!

Have you used Simple Plex Ultra-Sensitive Human NF-L Assay?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card