SIRT6 Antibody [FITC]

Novus Biologicals | Catalog # NB100-2523F

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Polyclonal Rabbit IgG
View all SIRT6 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an internal region of the human SIRT6 protein (within residues 250-350). [Swiss-Prot Q8N6T7]

Reactivity Notes

Human. Immunogen sequence has 78% homology to mouse protein and 71% homology to rat.

Localization

Nuclear

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Applications for SIRT6 Antibody [FITC]

Application
Recommended Usage

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This SIRT6 antibody is useful for Western blot analysis, where a band is seen at ~39 kDa. A higher MW band ~59 kDa may be seen with lower dilutions (ie: 2 ug/ml). This antibody is not applicable for Immunocytochemistry / Immunofluorescence. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: SIRT6

SIRT6 (SIR2-like protein 6) is a member of the yeast silent information regulator 2 (SIR2) family and possesses both strong ADP-ribosyltransferase activity as well as protein deacetylase activity which is dependent on NAD(+). SIRT6 exerts deacetylase activity towards histone H3K9Ac and H3K56Ac, and alters histone H3 acetylation in telomeric chromatin during cell cycle's S-phase. It deacetylates histone H3K9Ac at NFkB target promoters and downregulates a subset of NFkB target genes. Upon DNA damage, SIRT6 promotes DNA end resection via deacetylation of RBBP8. SIRT6 also serves as a corepressor of the transcription factor HIF1 alpha for regulating the expression of multiple glycolytic genes to control glucose homeostasis, and is required for normal IGF1 serum levels. SIRT6 play critical roles in maintaining telomere integrity, fine-tuning aging-associated gene expression programs, preventing genomic instability, and maintaining metabolic homeostasis, thus impacting on several pathways relevant for cancer, metabolism, aging, cellular senescence and apoptosis.

Long Name

NAD-dependent protein deacylase sirtuin-6

Alternate Names

EC 2.3.1.286, hSIRT6, SIR2-like protein 6, SIR2L6

Gene Symbol

SIRT6

Additional SIRT6 Products

Product Documents for SIRT6 Antibody [FITC]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for SIRT6 Antibody [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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