SIT1 Antibody (SIT-01) - BSA Free

Novus Biologicals | Catalog # NB500-486

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow (Intracellular), Immunoprecipitation, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # SIT-01

Format

BSA Free
View all SIT1 Primary Antibodies »

Product Specifications

Immunogen

Bacterially produced recombinant intracellular fragment of human SIT.

Reactivity Notes

Weak cross-reactivity with mouse.

Specificity

The antibody SIT-01 reacts with SHP2-interacting transmembrane adaptor protein (SIT) expressed exclusively in lymphoid organs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for SIT1 Antibody (SIT-01) - BSA Free

Western Blot: SIT1 Antibody (SIT-01)BSA Free [NB500-486]

Western Blot: SIT1 Antibody (SIT-01)BSA Free [NB500-486]

Western Blot: SIT1 Antibody (SIT-01) [NB500-486] - Analysis of human Jurkat T cell line lysate (1% laurylmaltoside); non-reduced sample, immunostained by mAb SIT-01 and goat anti-mouse IgG (H+L)-HRP conjugate.
Flow Cytometry: SIT1 Antibody (SIT-01) - BSA Free [NB500-486]

Flow Cytometry: SIT1 Antibody (SIT-01) - BSA Free [NB500-486]

Flow Cytometry: SIT1 Antibody (SIT-01) [NB500-486] - Intracellular staining of human peripheral blood using anti-SIT (clone SIT-01) PE.

Applications for SIT1 Antibody (SIT-01) - BSA Free

Application
Recommended Usage

Flow (Intracellular)

1-5 ug/ml

Immunoprecipitation

1:50

Western Blot

1-2 ug/ml
Application Notes
This antibody is CyTOF ready.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SIT1

Negatively regulates TCR (T-cell antigen receptor)-mediated signaling in T-cells. Involved in positive selection of T-cells.

Long Name

Signaling Threshold Regulating Transmembrane Adaptor 1

Alternate Names

gp30/40

Entrez Gene IDs

54390 (Mouse)

Gene Symbol

SIT1

Additional SIT1 Products

Product Documents for SIT1 Antibody (SIT-01) - BSA Free

Certificate of Analysis

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Product Specific Notices for SIT1 Antibody (SIT-01) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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