SKAP55 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87836

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SKAP55 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SLTIPYEEDEEEEEKEETYDDIDGFDSPSCGSQCRPTILPGSVGIKEPTEEKEEEDIYEVLPDEEHDLEEDESGTRRKGVDYASYYQGLWDCHGDQPDELSFQRGDL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SKAP55 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: SKAP55 Antibody [NBP1-87836]

Immunocytochemistry/ Immunofluorescence: SKAP55 Antibody [NBP1-87836]

Immunocytochemistry/Immunofluorescence: SKAP55 Antibody [NBP1-87836] - Staining of human cell line RPTEC TERT1 shows localization to nucleoplasm, plasma membrane & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: SKAP55 Antibody [NBP1-87836]

Immunohistochemistry-Paraffin: SKAP55 Antibody [NBP1-87836]

Immunohistochemistry-Paraffin: SKAP55 Antibody [NBP1-87836] - Staining in human lymph node and skeletal muscle tissues using anti-SKAP1 antibody. Corresponding SKAP1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: SKAP55 Antibody [NBP1-87836]

Immunohistochemistry-Paraffin: SKAP55 Antibody [NBP1-87836]

Immunohistochemistry-Paraffin: SKAP55 Antibody [NBP1-87836] - Staining of human lymph node shows high expression.
Immunohistochemistry-Paraffin: SKAP55 Antibody [NBP1-87836]

Immunohistochemistry-Paraffin: SKAP55 Antibody [NBP1-87836]

Immunohistochemistry-Paraffin: SKAP55 Antibody [NBP1-87836] - Staining of human skeletal muscle shows low expression as expected.

Applications for SKAP55 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SKAP55

Src kinase-associated phosphoprotein of 55 kDa (SKAP55) is expressed as a constitutively tyrosine phosphorylated 55-kD adapter protein that positively regulates TCR signaling. SKAP55 has also been shown to enhance adhesion to fibronectin and ICAM1, colocalize with F-actin at the T cell-antigen-presenting cell synapse and promotes the clustering of LFA1 and TCR ligation. TCR engagement induces the translocation of SKAP55 to lipid rafts, where the interactions with Fyn take place. CD45 also plays a very important role in SKAP55/TCR-mediated signaling. SKAP55 involvement in the TCR signaling pathway is the subject of current research in murine as well as human models.

Long Name

Src Kinase-associated Phosphoprotein of 55 kDa

Alternate Names

SCAP1, SKAP1

Gene Symbol

SKAP1

Additional SKAP55 Products

Product Documents for SKAP55 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SKAP55 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SKAP55 Antibody - BSA Free

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Protocols

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