SLC16A3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-13033

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (90%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SLC16A3 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human SLC16A3. The exact sequence is proprietary.

Reactivity Notes

Rat (88%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for SLC16A3 Antibody - BSA Free

Western Blot: SLC16A3 Antibody [NBP3-13033]

Western Blot: SLC16A3 Antibody [NBP3-13033]

Western Blot: SLC16A3 Antibody [NBP3-13033] - Unboiled HeLa whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SLC16A3 antibody (NBP3-13033) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)
SLC16A3 Antibody - BSA Free

Western Blot: SLC16A3 Antibody [NBP3-13033] -

Boiled and unboiled HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with MCT4 antibody (NBP3-13033) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for SLC16A3 Antibody - BSA Free

Application
Recommended Usage

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SLC16A3

Monocarboxylates, such as lactate and pyruvate, play an integral role in cellular metabolism. Lactic acid is produced in large quantities as a result of glycolysis, which provides the majority of ATP to cells under normal physiological conditions. However, accumulation of lactic acid leads to a decrease in intracellular pH and cessation of glycolysis. In order for glycolysis to continue at a high rate, lactic acid must be transported out of the cell. This transport process is carried out by a family of monocarboxylate transporters (MCTs),which function as proton symports and are stereoselective for L-lactate.The MCT family consists of at least eight members, MCT1-8, which contain between 10-12 transmembrane-helical (TM) domains, with the amino and carboxy termini located in the cytoplasm. MCT1 is widely expressed and is the major form of MCTs in tumor cells and erythrocytes. MCT2 is highly expressed in liver and testis, while MCT3 and MCT4 are predominantly expressed in skeletal muscle.

Long Name

Solute Carrier Family 16 Member 3

Alternate Names

MCT3, MCT4

Gene Symbol

SLC16A3

Additional SLC16A3 Products

Product Documents for SLC16A3 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SLC16A3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

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