Human SLC16A3 - Ready-To-Use ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP3-40073

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Tissue homogenates and other biological fluids (100 uL)

Sensitivity

0.28 ng/mL (example only; lot dependent)

Assay Range

0.78 - 50 ng/mL (example only; lot dependent)
View all SLC16A3 ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Description

The Ready-To-Use ELISA kit offers pre-diluted detection reagents and a shorter experimental time.
Assay Length: 3 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV 12 (example only; lot dependent)

Scientific Data Images for Human SLC16A3 - Ready-To-Use ELISA Kit (Colorimetric)

Human SLC16A3 - Ready-To-Use ELISA Kit (Colorimetric)

ELISA: Human SLC16A3 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-40073]

ELISA: Human SLC16A3 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-40073] - Standard Curve Reference

Kit Contents for Human SLC16A3 - Ready-To-Use ELISA Kit (Colorimetric)

  • Detection Solution A
  • Detection Solution B
  • Instruction manual
  • Plate sealer for 96 wells
  • Pre-coated 96T strip plate
  • Standard
  • Standard Diluent
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: SLC16A3

Monocarboxylates, such as lactate and pyruvate, play an integral role in cellular metabolism. Lactic acid is produced in large quantities as a result of glycolysis, which provides the majority of ATP to cells under normal physiological conditions. However, accumulation of lactic acid leads to a decrease in intracellular pH and cessation of glycolysis. In order for glycolysis to continue at a high rate, lactic acid must be transported out of the cell. This transport process is carried out by a family of monocarboxylate transporters (MCTs),which function as proton symports and are stereoselective for L-lactate.The MCT family consists of at least eight members, MCT1-8, which contain between 10-12 transmembrane-helical (TM) domains, with the amino and carboxy termini located in the cytoplasm. MCT1 is widely expressed and is the major form of MCTs in tumor cells and erythrocytes. MCT2 is highly expressed in liver and testis, while MCT3 and MCT4 are predominantly expressed in skeletal muscle.

Long Name

Solute Carrier Family 16 Member 3

Alternate Names

MCT3, MCT4

Gene Symbol

SLC16A3

Additional SLC16A3 Products

Product Documents for Human SLC16A3 - Ready-To-Use ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Human SLC16A3 - Ready-To-Use ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Related Research Areas

Cancer Biomarkers

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