SLC39A5 Antibody - Azide and BSA Free

Western Blot: SLC39A5 Antibody - Azide and BSA Free [NBP3-04949] -

Glucose is the main driver of sucrose-induced dysregulation of intestinal epithelial barrier function. (A) Following 24 h of glucose (G), fructose (F), and GF treatments, FITC-Dextran (green) fluorescence intensity within the enteroid was quantified by software ImageJ. Values are means +/- SEM; # of enteroid counted: mock, n = 322; GF, n = 293; G, n = 278; F, n = 253. One-way ANOVA. (B) Representative western analyses from two independent experiments showed ZIP14, ZIP4, and ZIP5 transporters. (C) Cellular location of ZIP14 at the basalateral side of the intestinal epithelium was shown in the mouse enteroid system. Images were obtained using Zeiss LSM880 confocal inverted microscope. Green: DAPI; red: ZIP14. (D) Depiction of the proposed mechanism of G or F-induced permeability. (E) Following 24 h of glucose (G) alone or combined with zinc treatments, FITC-Dextran (green) fluorescence intensity within the enteroid was quantified by software ImageJ. Values are means +/- SEM; # of enteroid counted: (G), n = 102; G + zinc (Zn), n = 111. (F) Zinc levels in intestinal epithelial cells were measured by MP-AES. (G,H) Intestinal permeability was assessed by FITC-Dextran and western blot analysis for TJP. Following 8 weeks of sucrose treatment the body weight (I) % body fat (J) and blood glucose levels were measured from floxed control and intestine-specific Zip14 KO (IKO-ZIP14 is deleted from villin-expressing cells) mice. Values are means +/- SEM. n = 6–8 mice per group. The unpaired t-test between sucrose-treated floxed and IKO mice. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37637953), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: SLC39A5 Antibody - Azide and BSA Free [NBP3-04949] -

Subchronic liquid sucrose treatment alters intestinal zinc metabolism. (A) Zinc concentration in intestinal epithelial cells (IEC) using MP-AES. IECs were separated and digested in nitric acid. Total protein concentrations were used for normalization. (B,C) Following the morning fast, mice were administered 65Zn via either gavage (B) or subcutaneous injection (C). Three hours later, the amount of radioactivity in intestine tissue was measured. (D) Representative western analyses show intestinal ZIP5 and ZIP14 protein levels from control and sucrose-fed mice (n = 3 mice per group). (E) Depiction of proposed intestinal zinc transporter and zinc regulation based on the data in A–D. Values are means +/- SEM; n = 4–7. Unpaired t-test between control and sucrose-treated groups. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37637953), licensed under a CC-BY license. Not internally tested by Novus Biologicals.