SLP-76/LCP2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-29836

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SLP-76/LCP2 Primary Antibodies »

Product Specifications

Immunogen

Between 200 and 250

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SLP-76/LCP2 Antibody - BSA Free

SLP-76/LCP2 Antibody

Immunoprecipitation:SLP-76/LCP2 AntibodyNBP3-29836] -

Immunoprecipitation:SLP-76/LCP2 AntibodyNBP3-29836] -Detection of human LCP2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-LCP2 antibody NBP3-29836 (lot NBP3-29836-1) used for IP at 6 µg per reaction. LCP2 was also immunoprecipitated by rabbit anti-LCP2.. For blotting immunoprecipitated LCP2, NBP3-29836 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for SLP-76/LCP2 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Tris-citrate/phosphate pH 7 -8.

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SLP-76/LCP2

SLP76 (SH2 domain containing leukocyte protein of 76 kDa) is a hematopoietic cell-specific adaptor protein that is crucial for T-cell receptor (TCR) signaling, hemostasis and platelet function. TCR ligation and fibrinogen binding to integrin alpha 2 beta 3 stimulates the phosphorylation of the tyrosine residues in the amino terminus, and facilitates SLP76 binding to the SH2 domain of Vav, which can activate JNK. SLP76 also comprises a proline-rich domain region that associates with the SH3 domain of Grb2 linking SLP76 to the Ras to Raf to ERK1 & 2 signaling pathway, LAT, PLC gamma, Fyn-binding protein (SLAP130), the SH2 containing phosphatase 1 and Nck, which mediates the regulation of cytoskeletal actin polymerization. Phosphorylation of tyrosine 145 has been shown to be important for optimal SLP76 function.

Long Name

SH2 Domain-containing Leukocyte Protein of 76 kDa

Alternate Names

LCP2, SLP76

Gene Symbol

LCP2

Additional SLP-76/LCP2 Products

Product Documents for SLP-76/LCP2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SLP-76/LCP2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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