Human SMAC/Diablo - Ready-To-Use ELISA Kit (Colorimetric)

Name: Human SMAC/Diablo - Ready-To-Use ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP3-31217
Price: 779 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-31217

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Product Documents

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Product Specifications
Precision
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Sample Type & Volume Required Per Well

Tissue homogenates, cell lysates and other biological fluids (100 uL)

Sensitivity

0.058 ng/mL (example only; lot dependent)

Assay Range

0.156 - 10 ng/mL (example only; lot dependent)

View all SMAC/Diablo ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Description

The Ready-To-Use ELISA kit offers pre-diluted detection reagents and a shorter experimental time.
Assay Length: 3 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV 12 (example only; lot dependent)

Scientific Data Images for Human SMAC/Diablo - Ready-To-Use ELISA Kit (Colorimetric)

ELISA: Human SMAC/Diablo - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31217] -

ELISA: Human SMAC/Diablo - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31217] - Standard Curve Reference

Kit Contents for Human SMAC/Diablo - Ready-To-Use ELISA Kit (Colorimetric)

Detection Solution A
Detection Solution B
Instruction manual
Plate sealer for 96 wells
Pre-coated 96T strip plate
Standard
Standard Diluent
Stop Solution
TMB Substrate
Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: SMAC/Diablo

Smac/DIABLO is a mitochondrial protein which activates various forms of apoptosis. Specifically, Smac exits the mitochondria and enters the cytosol where it initiates apoptosis by neutralizing members of the IAP family of apoptosis inhibitory proteins. Mitochondrial-mediated apoptosis is important in animal development and tissue homeostasis, with alterations resulting in a range of malignant disorders. Upon apoptotic stimuli, the mitochondrial proteins cytochrome c and Smac/DIABLO are released into the cytosol. The release of these proteins, however, occurs via different mechanisms.

Long Name

Second Mitochondria-derived Activator of Caspase

Alternate Names

Diablo

Gene Symbol

DIABLO

Additional SMAC/Diablo Products

Product Documents for Human SMAC/Diablo - Ready-To-Use ELISA Kit (Colorimetric)

Download Product Datasheets

Download Human SMAC/Diablo - Ready-To-Use ELISA Kit (Colorimetric) Manual

Download SDS

Product Specific Notices for Human SMAC/Diablo - Ready-To-Use ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Related Research Areas

Bcl-2 Proteins and Regulators

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