Smad1 [p Ser463, p Ser465] Antibody (S07-4A3)

Name: Smad1 [p Ser463, p Ser465] Antibody (S07-4A3)
Brand: Novus Biologicals
SKU: NBP3-15092
Price: 479 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-15092

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S07-4A3 expressed in HEK293

View all Smad1 Primary Antibodies »

Product Specifications

Immunogen

A synthetic phosphopeptide corresponding to residues surrounding Ser463/Ser465 of human Smad1 (Uniprot#: Q15797)

Modification

p Ser 463, p Ser 465

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Smad1 [p Ser463, p Ser465] Antibody (S07-4A3)

Western Blot: Smad1 [p Ser463, p Ser465] Antibody (S07-4A3) [NBP3-15092] - Western blot detection of Smad1 [p Ser463, p Ser465] in A549, U2OS, C6 cell lysates using Smad1 [p Ser463, p Ser465] Rabbit mAb (1:1000 diluted). Predicted band size: 52kDa. Observed band size: 52kDa.

Applications for Smad1 [p Ser463, p Ser465] Antibody (S07-4A3)

Application

Recommended Usage

Western Blot

1/1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Smad1

Smad1 is encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed.

Long Name

Mothers Against DPP Homolog 1

Alternate Names

BSP1, BSP-1, hSMAD1, JV4-1SMAD, mothers against DPP homolog 1 (Drosophila), MAD homolog 1, MADH1JV41, MADR1MAD, mothers against decapentaplegic homolog 1 (Drosophila), Mad-related protein 1, mothers against decapentaplegic homolog 1, Mothers against DPP homolog 1, SMAD family member 1SMAD 1, SMAD, mothers against DPP homolog 1, Smad1, TGF-beta signaling protein 1, transforming growth factor-beta signaling protein 1

Gene Symbol

SMAD1

Additional Smad1 Products

Product Documents for Smad1 [p Ser463, p Ser465] Antibody (S07-4A3)

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Certificate of Analysis

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Product Specific Notices for Smad1 [p Ser463, p Ser465] Antibody (S07-4A3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.