Q: I was wondering if you can recommend any primary polyclonal Smad3 antibody (reactivity: human) for gel supershifts?

A: We do provide a wide range of Smad3 antibodies, but unfortunately none of them have been tested for gel shift assay, so we can't guarantee they will work for an application never tested before.

Smad3 Antibody (BLR144J)

Novus Biologicals | Catalog # NBP3-14724

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit Clone # BLR144J

View all Smad3 Primary Antibodies »

Product Specifications

Immunogen

between 200 and 250

Clonality

Monoclonal

Host

Rabbit

Scientific Data Images for Smad3 Antibody (BLR144J)

Immunoprecipitation: Smad3 Antibody (BLR144J) [NBP3-14724]

Immunoprecipitation: Smad3 Antibody [NBP3-14724] - Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from PANC-1 cellsprepared using NETN lysis buffer. Antibodies: Rabbit antiSMAD3 recombinant monoclonal antibody used for IP at 20 ul/mg lysate. SMAD3was also immunoprecipitated by rabbit anti-SMAD3antibody. For blotting immunoprecipitatedSMAD3, was used at 1:1000.Chemiluminescence with an exposure time of 10 seconds.

Smad3 Antibody [NBP3-14724] -

Whole cell lysate (50 µg) from PANC-1, HeLa, A-549,Jurkat, HEK293T, K-562, U2OS, and HT-29 cells preparedusing NETN lysis buffer. Antibody: Rabbit anti-SMAD3recombinant monoclonal antibody [BLR144J] used at 1:1000. Secondary: HRP-conjugated goat antirabbit IgG.Chemiluminescence with anexposure time of 3 minutes. Lower Panel: Rabbit antiCOPB2 antibody.

Applications for Smad3 Antibody (BLR144J)

Application

Recommended Usage

Immunoprecipitation

15 µl/1 mg lysate

Western Blot

1:1,000

Formulation, Preparation, and Storage

Purification

>95%

Formulation

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: Smad3

The protein encoded by the Smad3 gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. (provided by RefSeq)

Long Name

Mothers Against DPP Homolog 3

Alternate Names

DKFZp586N0721, DKFZp686J10186, hMAD-3, hSMAD3, HsT17436, JV15-2MGC60396, MAD homolog 3, mad protein homolog, MAD, mothers against decapentaplegic homolog 3, MAD, mothers against decapentaplegic homolog 3 (Drosophila), mad3, MADH3mad homolog JV15-2, mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, SMA- and MAD-related protein 3, SMAD 3, SMAD family member 3HSPC193, SMAD, mothers against DPP homolog 3, SMAD, mothers against DPP homolog 3 (Drosophila), Smad3

Gene Symbol

SMAD3

Additional Smad3 Products

Product Documents for Smad3 Antibody (BLR144J)

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Certificate of Analysis

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Product Specific Notices for Smad3 Antibody (BLR144J)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Notch Pathway

Regulatory T Cells (Tregs)

Transcription Factors in the Akt Pathway

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