SMC2 Antibody - BSA Free
Novus Biologicals | Catalog # NB100-373
![Western Blot: SMC2 Antibody [NB100-373]](https://resources.rndsystems.com/images/products/SMC2-Antibody-Western-Blot-NB100-373-img0014.jpg "Western Blot: SMC2 Antibody [NB100-373]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockdown Validated
Cited:
Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockdown Validated
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 1150 and the C-terminus (residue 1197) of human Structural Maintenance of Chromosomes 2 using the numbering given in entry NP_006435.1 (GeneID 10592).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SMC2 Antibody - BSA Free
Western Blot: SMC2 Antibody [NB100-373]
Western Blot: SMC2 Antibody [NB100-373] - Detection of Human SMC2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMC2 antibody NB100-373 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.![Immunocytochemistry/ Immunofluorescence: SMC2 Antibody [NB100-373]](https://resources.rndsystems.com/images/products/SMC2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-373-img0015.jpg "Immunocytochemistry/ Immunofluorescence: SMC2 Antibody [NB100-373]")
![Western Blot: SMC2 Antibody [NB100-373]](https://resources.rndsystems.com/images/products/SMC2-Antibody-Western-Blot-NB100-373-img0001.jpg "Western Blot: SMC2 Antibody [NB100-373]")
Western Blot: SMC2 Antibody [NB100-373]
Western Blot: SMC2 Antibody [NB100-373] - Detection of Human SMC2 on HeLa whole cell lysate using NB100-373.![Western Blot: SMC2 Antibody [NB100-373]](https://resources.rndsystems.com/images/products/SMC2-Antibody-Western-Blot-NB100-373-img0012.jpg "Western Blot: SMC2 Antibody [NB100-373]")
Western Blot: SMC2 Antibody [NB100-373]
Western Blot: SMC2 Antibody [NB100-373] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibod ies : Affinity purified rabbit anti-SMC2 antibody (lot was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Immunocytochemistry/ Immunofluorescence: SMC2 Antibody - BSA Free [NB100-373] -
SMC2 (a) and SMC4 (b) in HaCaT keratinocytes treated with control shRNA (upper panels) and treated with Nesprin-2-SMC domain specific shRNA (lower panels). Nesprin-2 was detected with mAb K20-478. Bar, 10 μm. (c) Localization of Nesprin-2 after siRNA mediated knockdown of SMC2 in COS7 cells. Staining was with SMC2 specific antibodies and mAb K20-478 for Nesprin-2. Bar, 5 μm. (d) Evaluation of the SMC2 knockdown. SMC2 fluorescence intensity was measured in the center of mitotic chromosomes. 10 siRNA treated cells and 12 control cells (control treatment) were analyzed (∗∗∗P value = 0.0001). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29445399), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for SMC2 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:200-1:400
Immunoprecipitation
2-10 ug/mg lysate
Western Blot
1:2000 - 1:10000
Application Notes
NB 100-373 may be used for Western Blot, where a band at ~155 kDa is seen, representing SMC2 protein. *The investigator should determine the optimal working dilution for a specific application.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: SMC2
Alternate Names
CAP-E, CAPESMC2 structural maintenance of chromosomes 2-like 1 (yeast), chromosome-associated protein E, FLJ10093, hCAP-EXCAP-E homolog, SMC protein 2, SMC-2, SMC2 (structural maintenance of chromosomes 2, yeast)-like 1, SMC2 structural maintenance of chromosomes 2-like 1, SMC2L1, structural maintenance of chromosomes (SMC) family member, structural maintenance of chromosomes 2, structural maintenance of chromosomes protein 2
Entrez Gene IDs
10592 (Human)
Gene Symbol
SMC2
UniProt
Additional SMC2 Products
Product Documents for SMC2 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for SMC2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for SMC2 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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