Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockdown Validated

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SMC2 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1150 and the C-terminus (residue 1197) of human Structural Maintenance of Chromosomes 2 using the numbering given in entry NP_006435.1 (GeneID 10592).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SMC2 Antibody - BSA Free

Western Blot: SMC2 Antibody [NB100-373]

Western Blot: SMC2 Antibody [NB100-373]

Western Blot: SMC2 Antibody [NB100-373] - Detection of Human SMC2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMC2 antibody NB100-373 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Immunocytochemistry/ Immunofluorescence: SMC2 Antibody [NB100-373]

Immunocytochemistry/ Immunofluorescence: SMC2 Antibody [NB100-373]

SMC2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-373-img0015.jpg
Western Blot: SMC2 Antibody [NB100-373]

Western Blot: SMC2 Antibody [NB100-373]

Western Blot: SMC2 Antibody [NB100-373] - Detection of Human SMC2 on HeLa whole cell lysate using NB100-373.
Western Blot: SMC2 Antibody [NB100-373]

Western Blot: SMC2 Antibody [NB100-373]

Western Blot: SMC2 Antibody [NB100-373] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibod ies : Affinity purified rabbit anti-SMC2 antibody (lot was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
SMC2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: SMC2 Antibody - BSA Free [NB100-373] -

SMC2 (a) and SMC4 (b) in HaCaT keratinocytes treated with control shRNA (upper panels) and treated with Nesprin-2-SMC domain specific shRNA (lower panels). Nesprin-2 was detected with mAb K20-478. Bar, 10 μm. (c) Localization of Nesprin-2 after siRNA mediated knockdown of SMC2 in COS7 cells. Staining was with SMC2 specific antibodies and mAb K20-478 for Nesprin-2. Bar, 5 μm. (d) Evaluation of the SMC2 knockdown. SMC2 fluorescence intensity was measured in the center of mitotic chromosomes. 10 siRNA treated cells and 12 control cells (control treatment) were analyzed (∗∗∗P value = 0.0001). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29445399), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for SMC2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:200-1:400

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000 - 1:10000
Application Notes
NB 100-373 may be used for Western Blot, where a band at ~155 kDa is seen, representing SMC2 protein. *The investigator should determine the optimal working dilution for a specific application.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SMC2

Structural Maintenance of Chromosome (SMC) proteins are required for nuclear processes, including chromosome condensation, chromatid cohesion, and DNA repair. Eukaryotic proteins are capable of binding DNA and possess a DNA-stimulated ATPase activity.

Alternate Names

CAP-E, CAPESMC2 structural maintenance of chromosomes 2-like 1 (yeast), chromosome-associated protein E, FLJ10093, hCAP-EXCAP-E homolog, SMC protein 2, SMC-2, SMC2 (structural maintenance of chromosomes 2, yeast)-like 1, SMC2 structural maintenance of chromosomes 2-like 1, SMC2L1, structural maintenance of chromosomes (SMC) family member, structural maintenance of chromosomes 2, structural maintenance of chromosomes protein 2

Entrez Gene IDs

10592 (Human)

Gene Symbol

SMC2

UniProt

NP_006435.1 (Human)

Additional SMC2 Products

Product Documents for SMC2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SMC2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SMC2 Antibody - BSA Free

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Protocols

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FAQs

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