Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SMC4 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 600 and 650 of human Structural Maintenance of Chromosomes 4 using the numbering given in entry NP_005487.2 (GeneID 10051).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SMC4 Antibody - BSA Free

Western Blot: SMC4 Antibody [NB100-374]

Western Blot: SMC4 Antibody [NB100-374]

Western Blot: SMC4 Antibody [NB100-374] - Detection of Human SMC4 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMC4 antibody NB100-374 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Immunocytochemistry/ Immunofluorescence: SMC4 Antibody [NB100-374]

Immunocytochemistry/ Immunofluorescence: SMC4 Antibody [NB100-374]

Immunocytochemistry/Immunofluorescence: SMC4 Antibody [NB100-374] - HeLa cells grown on coverslips, fixed with methanol/acetone and blocked with 3% BSA in PBS. Antibody used at 5 ug/ml. Detection: Rhodamine labeled anti-rabbit secondary antibody. Cells were counterstained with DAPI.
Western Blot: SMC4 Antibody [NB100-374]

Western Blot: SMC4 Antibody [NB100-374]

Western Blot: SMC4 Antibody [NB100-374] - Whole cell lysate (90 ug) from 293T cells (lane 1), SMC4 transfected 239T cells (lane 2) or normal human lymphoblasts (lane 3) separated on an 8% SDS-PAGE gel. Antibody used at 0.2 ug/ml and goat anti-rabbit HRP used at a dilution of 1:20,000.

Applications for SMC4 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500 - 1:2000

Western Blot

1:1000-1:10000
Application Notes
Immunofluorescence and Western Blot: The investigator should determine the optimal working dilution for a specific application.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SMC4

Structural Maintenance of Chromosome (SMC) proteins are required for nuclear processes, including chromosome condensation, chromatid cohesion, and DNA repair. Eukaryotic proteins are capable of binding DNA and possess a DNA-stimulated ATPase activity.

Alternate Names

CAP-C, CAPCSMC4 structural maintenance of chromosomes 4-like 1 (yeast), Chromosome-associated polypeptide C, hCAP-CSMC4 (structural maintenance of chromosomes 4, yeast)-like 1, SMC protein 4, SMC-4, SMC4 structural maintenance of chromosomes 4-like 1, SMC4L1, structural maintenance of chromosomes 4, structural maintenance of chromosomes protein 4, XCAP-C homolog

Entrez Gene IDs

10051 (Human)

Gene Symbol

SMC4

UniProt

NP_005487.2 (Human)

Additional SMC4 Products

Product Documents for SMC4 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SMC4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SMC4 Antibody - BSA Free

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Protocols

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