Smith Antigen ELISA Kit (Colorimetric)

Name: Smith Antigen ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: KA1112
Price: 569 USD
Availability: InStock

Novus Biologicals | Catalog # KA1112

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Product Specifications
Precision
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Plasma, Serum (100 uL (prediluted))

Sensitivity

1.0 U/mL

Assay Range

12.5 to 200 U/mL

View all Smith Antigen ELISA Kits »

Product Specifications

Assay Type

Indirect ELISA

Kit Type

ELISA Kit (Colorimetric)

Description

Sm Ab ELISA Kit is an indirect solid phase ELISA for the quantitative measurement of IgG class autoantibodies against Sm in human serum or plasma.

Precision

Intra-Assay Precision (Precision within an assay) CV% 2.0-4.1%

Inter-Assay Precision (Precision between assays) CV% 1.3-5.3%

Kit Contents for Smith Antigen ELISA Kit (Colorimetric)

Calibrators A-F (0, 12.5, 25, 50, 100, 200 U/mL), containing Sm antibodies in a serum/buffer matrix (PBS, BSA, detergent, NaN3 0.09%), yellow. Ready to use.
Control positive (1) and negative (2), containing Sm antibodies in a serum/buffer matrix (PBS, BSA, detergent, NaN3 0.09%), yellow. Ready to use. The concentration is specified on the certificate of analysis.
Divisible microplate consisting of 12 modules of 8 wells each. Ready to use.
Enzyme conjugate, containing anti-human IgG antibodies, HRP labelled; PBS, BSA, detergent, preservative Proclin 300 0.05%, light red. Ready to use.
Sample buffer P, containing PBS, BSA, detergent, preservative NaN3 0.09%, yellow, concentrate (5x).
Stop solution, contains acid. Ready to use.
TMB substrate, containing 3,3’,5,5’- Tetramethylbenzidine, colorless. Ready to use.
Wash solution, containing Tris, detergent, preservative NaN3 0.09%, 50x concentrate.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Smith Antigen

The sm (Smith) is an autoantigen on a series of particles composed of RNA and protein. Unlike RNP, the sm antigen is resistant to RNase, but partially sensitive to trypsin. It is involved in the pathogenesis of Systemic lupus erythematosus (SLE). Antibodies to sm are found in 20-30% of patients with SLE.

Alternate Names

Smith antigen

Additional Smith Antigen Products

Product Documents for Smith Antigen ELISA Kit (Colorimetric)

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Download Sm Ab ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Smith Antigen ELISA Kit (Colorimetric)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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