SOD1/Cu-Zn SOD Antibody

Novus Biologicals | Catalog # NB100-60944

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Canine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Peptide ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all SOD1/Cu-Zn SOD Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-SRKHGGPKDEERH corresponding to internal region according to NP_000445.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SOD1/Cu-Zn SOD Antibody

Western Blot: SOD1/Cu-Zn SOD Antibody [NB100-60944]

Western Blot: SOD1/Cu-Zn SOD Antibody [NB100-60944]

Western Blot: SOD1/Cu-Zn SOD Antibody [NB100-60944] - Staining of NIH3T3 (A), HEK293 (B), HepG2 (C) and MCF7 (D) lysates (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: SOD1/Cu-Zn SOD Antibody [NB100-60944]

Immunocytochemistry/ Immunofluorescence: SOD1/Cu-Zn SOD Antibody [NB100-60944]

Immunocytochemistry/Immunofluorescence: SOD1/Cu-Zn SOD Antibody [NB100-60944] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Western Blot: SOD1/Cu-Zn SOD Antibody [NB100-60944]

Western Blot: SOD1/Cu-Zn SOD Antibody [NB100-60944]

Western Blot: SOD1/Cu-Zn SOD Antibody [NB100-60944] - Staining of Mouse Brain lysate (35 ug protein in RIPA buffer). Antibody at 0.02 ug/mL. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: SOD1/Cu-Zn SOD Antibody [NB100-60944]

Immunocytochemistry/ Immunofluorescence: SOD1/Cu-Zn SOD Antibody [NB100-60944]

Immunocytochemistry/Immunofluorescence: SOD1/Cu-Zn SOD Antibody [NB100-60944] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Applications for SOD1/Cu-Zn SOD Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Peptide ELISA

Detection limit 1:8000

Western Blot

0.01 - 0.03 ug/mL
Application Notes
Western blot: Approx 18 kDa band observed in Mouse Brain and Rat Spinal Cord lysates, and in lysates of cell line NIH3T3. Approx 20 kDa observed in lysates of cell lines HEK293, HepG2 and MCF7 (calculated MW of 15.9 kDa according to Human NP_000445.1, Mouse NP_035564.1 and Rat NP_058746.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SOD1/Cu-Zn SOD

The protein encoded by this gene binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene.

Long Name

Superoxide Dismutase-1

Alternate Names

Cu-Zn SOD, CuZn SOD, Ipo1, IPOA, SOD, cytosolic, SOD, Soluble

Entrez Gene IDs

6647 (Human); 20655 (Mouse); 24786 (Rat)

Gene Symbol

SOD1

UniProt

P00441

Additional SOD1/Cu-Zn SOD Products

Product Documents for SOD1/Cu-Zn SOD Antibody

Certificate of Analysis

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Product Specific Notices for SOD1/Cu-Zn SOD Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SOD1/Cu-Zn SOD Antibody

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Protocols

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