Recombinant Mouse SOD2/Mn-SOD His Protein

Novus Biologicals | Catalog # NBP2-52079

Novus Biologicals
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Key Product Details

Source

E. coli

Tag

His

Applications

Western Blot, SDS-PAGE
View all SOD2/Mn-SOD Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids25-222 of Mouse SOD2/Mn-SOD

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSKHSLPDL PYDYGALEPH INAQIMQLHH SKHHAAYVNN LNATEEKYHE ALAKGDVTTQ VALQPALKFN GGGHINHTIF WTNLSPKGGG EPKGELLEAI KRDFGSFEKF KEKLTAVSVG VQGSGWGWLG FNKEQGRLQI AACSNQDPLQ GTTGLIPLLG IDVWEHAYYL QYKNVRPDYL KAIWNVINWE NVTERYTACK K

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

24.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Use in WB reported in secitific publication PMID: 32560467

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Mouse SOD2/Mn-SOD His Protein

SDS-PAGE: Recombinant Mouse SOD2/Mn-SOD His Protein [NBP2-52079]

SDS-PAGE: Recombinant Mouse SOD2/Mn-SOD His Protein [NBP2-52079]

SDS-Page: SOD2/Mn-SOD Recombinant Protein [NBP2-52079] - 15% SDS Page (3 ug)

Formulation, Preparation, and Storage

NBP2-52079
Formulation PBS (pH 7.4), 10% glycerol, 1 mM DTT
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SOD2/Mn-SOD

Superoxide dismutase (SOD) is responsible for the elimination of cytotoxic active oxygen by catalyzing the dismutation of the superoxide radical to oxygen and hydrogen peroxide (1, 2, 3). There are three SOD isoenzymes in mammalian cells. They are: extracellular SOD (EC SOD), copper and zinc-containing SOD (CuZn SOD) and manganese-containing SOD (Mn SOD, SOD-2). The CuZn form contains Cu and Zn ions and exists as a 32 kDa dimer in the cytosol. Mn SOD is an 80 kDa tetramer that contains Mn ion and resides in the mitochondrial matrix. Mn SOD is a tumor necrosis factor (TNF)-inducible enzyme that protects cells from NFmediated apoptosis (4) via superoxide anion detoxification and the subsequent regulation of apoptosis through cytochrome c release and the modulation of the redox state of the mitochondria (6). Mn SOD has also been shown to be a tumor suppressor in human breast cancer (7). Overexpression of this enzyme protects neurons from NMDAand nitric oxide-induced neurotoxicity.

Long Name

Superoxide Dismutase-2

Alternate Names

IPO-B, Mn SOD, MnSOD, SOD, Mitochodrial

Gene Symbol

SOD2

Additional SOD2/Mn-SOD Products

Product Documents for Recombinant Mouse SOD2/Mn-SOD His Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse SOD2/Mn-SOD His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

Citations for Recombinant Mouse SOD2/Mn-SOD His Protein

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