SPANXC Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-03285
![Western Blot: SPANXC AntibodyBSA Free [NBP1-03285]](https://resources.rndsystems.com/images/products/SPANXC-Antibody-Western-Blot-NBP1-03285-img0003.jpg "Western Blot: SPANXC AntibodyBSA Free [NBP1-03285]")
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Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Chimpanzee (90%), Primate (90%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Synthetic peptide corresponding to an internal region near aa 25-50 of human SPANXC.
Reactivity Notes
Predicted reactivity based on percent identity from BLAST analysis: Gorilla (90%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SPANXC Antibody - BSA Free
Western Blot: SPANXC AntibodyBSA Free [NBP1-03285]
Western Blot: SPANXC Antibody [NBP1-03285] - Affinity purified anti-SPANX (pan) antibody shows detection of a band at ~17 kD corresponding to SPANX-C present in a nuclear extract from VWM105 cells (left panel, arrowhead). VWM105 cells are derived from a human melanoma and are positive for SPANX proteins. Lane 2 shows reactivity with a purified recombinant SPANX-C fusion protein. The right panel shows similar reactivity with purified recombinant SPANX-B, SPANX-C and SPANX-N proteins. Proteins were separated by SDS-PAGE, transferred to nitrocellulose, and probed with the primary antibody diluted to 1:1000. IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX ( was used (left). IRDye is a trademark of LI-COR, Inc. Size estimation was made by comparison to prestained MW markers as indicated. Personal Communication. Vladimir Larionov, NIH, CCR, Bethesda, MD.![Immunohistochemistry-Paraffin: SPANXC Antibody - BSA Free [NBP1-03285]](https://resources.rndsystems.com/images/products/SPANXC-Antibody-Immunohistochemistry-Paraffin-NBP1-03285-img0001.jpg "Immunohistochemistry-Paraffin: SPANXC Antibody - BSA Free [NBP1-03285]")
Immunohistochemistry-Paraffin: SPANXC Antibody - BSA Free [NBP1-03285]
Immunohistochemistry-Paraffin: SPANXC Antibody [NBP1-03285] - Staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.Applications for SPANXC Antibody - BSA Free
Application
Recommended Usage
ELISA
1:10000-1:50000
Immunohistochemistry
5 ug/ml
Immunohistochemistry-Paraffin
5 ug/ml
Western Blot
1:500-1:3000
Application Notes
.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
20mM Potassium Phosphate (pH 7.2) and 0.15M NaCl
Format
BSA Free
Preservative
0.01% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SPANXC
Alternate Names
B1, C, cancer/testis antigen 11.3, cancer/testis antigen family 11, member 3, cancer/testis-associated protein CTp11, cancer/testis-associated protein of 11 kD, cancer-testis-associated protein CTp11, CT11.1, CT11.2, CT11.3, CT11.4, CTp11, dJ171K16.1, NAP-X, nuclear-associated protein SPAN-Xc, SPANX, SPANX family, member C, SPANXA, SPAN-Xa, SPANX-A, SPANXB, SPAN-Xb, SPANX-B, SPANX-C, SPAN-Xc protein, SPANX-D, sperm protein associated with the nucleus on the X chromosome C, sperm protein associated with the nucleus, X chromosome, family member C
Gene Symbol
SPANXC
UniProt
Additional SPANXC Products
Product Documents for SPANXC Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for SPANXC Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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