Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%). Backed by our 100% Guarantee.

Applications

Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1350 and 1400 of human SMART/HDAC1 associated repressor protein (spen homolog, transcriptional regulator) using the numbering given in entry NP_055816.2 (GeneID 23013).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SPEN Antibody - BSA Free

Immunoprecipitation: SPEN Antibody [NB100-58798]

Immunoprecipitation: SPEN Antibody [NB100-58798]

Immunoprecipitation: SPEN Antibody [NB100-58798] - Detection of human SHARP by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SHARP antibody NB100-58798 used for IP at 6 ug per reaction. For blotting immunoprecipitated SHARP, NB100-58798 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for SPEN Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2-10 ug/mg lysate
Application Notes
Western Blot - Not recommended for crude preparations. It can be used successfully for WB of enriched preparations (e.g. immunoprecipitated) SHARP.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SPEN

This gene encodes a hormone inducible transcriptional repressor. Repression of transcription by this gene product can occur through interactions with other repressors, by the recruitment of proteins involved in histone deacetylation, or through sequestration of transcriptional activators. The product of this gene contains a carboxy-terminal domain that permits binding to other corepressor proteins. This domain also permits interaction with members of the NuRD complex, a nucleosome remodeling protein complex that contains deacetylase activity. In addition, this repressor contains several RNA recognition motifs that confer binding to a steroid receptor RNA coactivator; this binding can modulate the activity of both liganded and nonliganded steroid receptors.

Alternate Names

HIAA0929, KIAA0929nuclear receptor transcription cofactor, MINTMsx2 interacting nuclear target (MINT) homolog, RBM15C, SHARPmsx2-interacting protein, SMART/HDAC1 associated repressor protein, SMART/HDAC1-associated repressor protein, SPEN homolog, spen homolog, transcriptional regulator (Drosophila)

Entrez Gene IDs

23013 (Human)

Gene Symbol

SPEN

UniProt

Q96T58 (Human)

Additional SPEN Products

Product Documents for SPEN Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SPEN Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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