SPRi 3

Tocris Bioscience | Catalog # 6597

Potent sepiapterin reductase (SPR) inhibitor; attenuates proliferation of CD4+ T cells
Tocris Bioscience

Key Product Details

Description

Potent sepiapterin reductase (SPR) inhibitor; attenuates proliferation of CD4+ T cells
View all Other Reductase Inhibitors »

Product Description

SPRi 3 is a potent sepiapterin reductase (SPR) inhibitor (IC50 = 53 - 74 nM for human SPR). Inhibits BH4 synthesis pathway and attenuates proliferation in naive CD4+ T cells. Suppresses proliferation of human effector CD4+ T cells following stimulation. Reduces SPR activity in mouse primary cultures of sensory neurons (IC50 = 450 nM). Ameliorates colitis, suppressing the intestinal infiltration of T cells and other immune cells in vivo. Also reduces immune-cell infiltration into mouse lungs. Reduces neuropathic and inflammatory pain in mouse models.

Licensing Information

Sold under license from the Children's Medical Center Corporation.

Product Specifications for SPRi 3

Molecular Weight

262.30

Formula

C14H18N2O3

Storage

Store at -20°C

Purity

≥98% (HPLC)

Chemical Name

N-[2-(5-Hydroxy-2-methyl-1H-indol-3-yl)ethyl]-2-methoxyacetamide

CAS Number

1292285-54-1

PubChem ID

52911386

InChI Key

YBXBWBBVLXZQBJ-UHFFFAOYSA-N

SMILES

COCC(NCCC1=C(NC2=CC=C(C=C12)O)C)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 26.23 100
Ethanol 26.23 100

Preparing Stock Solutions for SPRi 3

The following data is based on the product molecular weight 262.30.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.81 mL 19.06 mL 38.12 mL
5 mM 0.76 mL 3.81 mL 7.62 mL
10 mM 0.38 mL 1.91 mL 3.81 mL
50 mM 0.08 mL 0.38 mL 0.76 mL

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Background References

References are publications that support the biological activity of the product.

Product Documents for SPRi 3

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for SPRi 3

For research use only

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  • SPR inhibitor
    Name: Anonymous
    Species: Rat
    Assay Type: In Vitro
    Cell Line/Tissue: Primary hippocampal neurons
    Verified Customer | Posted 09/26/2025
    The drug was incubated at 1 μM to inhibit Sepiapterin Reductase function.

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