![Western Blot: SPRY4 Antibody [NBP2-20479]](https://resources.rndsystems.com/images/products/SPRY4-Antibody-Western-Blot-NBP2-20479-img0002.jpg "Western Blot: SPRY4 Antibody [NBP2-20479]")
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Key Product Details
Species Reactivity
Validated:
Human, Mouse
Predicted:
Bovine (95%), Rat (94%), Sheep (94%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human SPRY4. The exact sequence is proprietary.
Localization
Cytoplasm; Cell projection; ruffle membrane; Peripheral membrane protein; Cytoplasmic side
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for SPRY4 Antibody
Western Blot: SPRY4 Antibody [NBP2-20479]
Western Blot: SPRY4 Antibody [NBP2-20479] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 12% SDS PAGE gel, diluted at 1:1000.![Immunohistochemistry-Paraffin: SPRY4 Antibody [NBP2-20479]](https://resources.rndsystems.com/images/products/SPRY4-Antibody-Immunohistochemistry-Paraffin-NBP2-20479-img0004.jpg "Immunohistochemistry-Paraffin: SPRY4 Antibody [NBP2-20479]")
Immunohistochemistry-Paraffin: SPRY4 Antibody [NBP2-20479]
Immunohistochemistry-Paraffin: SPRY4 Antibody [NBP2-20479] - Paraffin-embedded mouse duodenum. SPRY4 antibody diluted at 1:500.![Immunohistochemistry-Paraffin: SPRY4 Antibody [NBP2-20479]](https://resources.rndsystems.com/images/products/SPRY4-Antibody-Immunohistochemistry-Paraffin-NBP2-20479-img0003.jpg "Immunohistochemistry-Paraffin: SPRY4 Antibody [NBP2-20479]")
Immunohistochemistry-Paraffin: SPRY4 Antibody [NBP2-20479]
Immunohistochemistry-Paraffin: SPRY4 Antibody [NBP2-20479] - Paraffin-embedded U87 xenograft. SPRY4 antibody dilution: 1:500.
Western Blot: SPRY4 Antibody [NBP2-20479] -
Western Blot: SPRY4 Antibody [NBP2-20479] - SPRY4 antibody detects SPRY4 protein by Western blot analysis.A. 30 ug NT2D1 whole cell lysate/extract
12 % SDS-PAGE
SPRY4 antibody (NBP2-20479) dilution: 1:500
Applications for SPRY4 Antibody
Application
Recommended Usage
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: SPRY4
Long Name
Sprouty Homolog 4
Alternate Names
protein sprouty homolog 4, sprouty homolog 4 (Drosophila), Spry-4
Gene Symbol
SPRY4
UniProt
Additional SPRY4 Products
Product Documents for SPRY4 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for SPRY4 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.Customer Reviews for SPRY4 Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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