SSEA-1 Antibody (MEM-158) [FITC]

Novus Biologicals | Catalog # NB500-377

Novus Biologicals

Key Product Details

Species Reactivity

Human, Bovine (Negative), Porcine (Negative), Sheep (Negative)

Applications

Flow Cytometry

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Monoclonal Mouse IgM Clone # MEM-158
View all SSEA-1 Primary Antibodies »

Product Specifications

Immunogen

Human granulocytes

Localization

Cell Membrane

Specificity

The antibody MEM-158 reacts with CD15, a cell membrane molecule 3-fucosyl-N-acetyllactosamine (3-FAL) strongly expressed on granulocytes, monocytes, macrophages, mast cells; it is also present on Langerhans cells and some myeloid precursors cells. HLDA VI; WS Code AS A053

Clonality

Monoclonal

Host

Mouse

Isotype

IgM

Scientific Data Images for SSEA-1 Antibody (MEM-158) [FITC]

Flow Cytometry: CD15/Lewis X Antibody (MEM-158) [FITC] [NB500-377] - Fig. 1. Surface staining of human peripheral blood cells with anti-human CD15 (MEM-158) FITC. Cells in the granulocyte gate were used for analysis.

Applications for SSEA-1 Antibody (MEM-158) [FITC]

Application
Recommended Usage

Flow Cytometry

20 ul/100 ul of whole blood or 10^6 cells in suspension

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Size Exclusion Chromatography

Formulation

Tris buffered saline (TBS), pH 8.0

Preservative

15mM Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: SSEA-1

CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin's disease.

Long Name

Stage-specific Embryonic Antigen-1

Alternate Names

SSEA1

Gene Symbol

FUT4

Additional SSEA-1 Products

Product Documents for SSEA-1 Antibody (MEM-158) [FITC]

Certificate of Analysis

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Product Specific Notices for SSEA-1 Antibody (MEM-158) [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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