STAG2 Antibody

Novus Biologicals | Catalog # NBP1-30472

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Cited:

Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all STAG2 Primary Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 1 and 50 of human stromal antigen 2 using the numbering given in entry NP_006594.3 (GeneID 10735).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

141 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for STAG2 Antibody

Western Blot: STAG2 Antibody [NBP1-30472]

Western Blot: STAG2 Antibody [NBP1-30472]

Western Blot: STAG2 Antibody [NBP1-30472] - Western Blot and Immunoprecipitation: Whole cell lysate from HeLa and 293T cells. SA2 was also immunoprecipitated by rabbit anti-SA2 antibody NBP1-30473.
Immunohistochemistry-Paraffin: STAG2 Antibody [NBP1-30472]

Immunohistochemistry-Paraffin: STAG2 Antibody [NBP1-30472]

Immunohistochemistry-Paraffin: STAG2 Antibody [NBP1-30472] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- SA2 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB

Applications for STAG2 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:500 - 1:2000

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: STAG2

SA2 is a component of the cohesin complex, which is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which, sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. The subunit interacts directly with RAD21 in cohesin complex. Cohesin complexes are composed of a heterodimer between a SMC1 protein (SMC1L1 or SMC1L2) and SMC3, which are attached via their hinge domain, and RAD21 links them at their heads, and one STAG protein (STAG1, STAG2 or STAG3). In cohesin complexes, STAG2 is mutually exclusive with STAG1 and STAG3.

Alternate Names

bA517O1.1, cohesin subunit SA-2, DKFZp686P168, SA-2, SA2FLJ25871, SCC3 homolog 2, stromal antigen 2DKFZp781H1753

Entrez Gene IDs

10735 (Human)

Gene Symbol

STAG2

UniProt

Q8N3U4 (Human)

Additional STAG2 Products

Product Documents for STAG2 Antibody

Certificate of Analysis

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Product Specific Notices for STAG2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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