StemXVivo Cardiomyocyte Maintenance Media Supplement (50X)

R&D Systems | Catalog # AR011

R&D Systems
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Product Summary for StemXVivo Cardiomyocyte Maintenance Media Supplement (50X)

Kit Summary

A serum-free cell culture media supplement for the extended culture of cardiomyocytes.

Key Benefits

  • Optimized for the extended culture of cardiomyocytes derived from pluripotent stem cells
  • Maintains a healthy cardiomyocyte population in culture for longer than 200 days
  • Is nutrient-rich, fully defined, and serum-free
  • High lot-to-lot consistency decreases variation
 

Why Culture Cardiomyocytes in a Defined Medium?

Maintaining the health of pluripotent stem cell-derived cardiomyocytes is imperative for generating trustworthy data during downstream experimentation. Culturing cardiomyocytes in serum-free, defined media reduces experimental variability in cell health by eliminating the uncontrolled variables that are often associated with serum-containing media, such as indeterminate levels of vitamins, hormones, and growth factors.

Cardiomyocytes cultured using the Cardiomyocyte Maintenance Media Supplement can also be a part of drug toxicity and small molecule screening workflows where a controlled cell culture environment is essential.

Cardiomyocyte Maintenance Media Use

Human pluripotent stem cells are differentiated into cardiomyocytes using the StemXVivo® Cardiomyocyte Differentiation Kit (R&D Systems, Catalog # SC032B) until widespread beating is observed in the culture. StemXVivo® Cardiomyocyte Maintenance Media can then be used to maintain the differentiated cells for extended culture. Media should be replaced daily.

Note: Repeated mechanical stress can negatively affect cell attachment and survival. For extended culture, daily media exchanges should be handled as gently as possible.

Precautions

This product contains human transferrin. This transferrin was purified from donor plasma and tested at the donor level using an FDA licensed method and found to be non-reactive for anti-HIV-1/2 and Hepatitis B surface antigen. 

Scientific Data Images for StemXVivo Cardiomyocyte Maintenance Media Supplement (50X)

Detection of Cardiac Troponin T in Human ES-derived Cardiomyocytes.

Detection of Cardiac Troponin T in Human ES-derived Cardiomyocytes.

Detection of Cardiac Troponin T in Human ES-derived Cardiomyocytes.BG01V human embryonic stem cells were differentiated into cardiomyocytes using the StemXVivo®Cardiomyocyte Differentiation Kit (Catalog # SC032B) and maintained in StemXVivo®Cardiomyocyte Maintenance Media Supplement (Catalog # AR011) until day 80 of differentiation. Cardiac Troponin T (red) was detected in cardiomyocytes with a Mouse Anti-Human Troponin T (Cardiac) Monoclonal Antibody (Catalog # MAB1874) and a NorthernLights™ (NL)557-conjugated Donkey Anti-Mouse Affinity Purified Polyclonal Antibody (Catalog # NL007). The nuclei were counterstained with DAPI (blue).
Troponin I Expression in Human ES-derived Cardiomyocytes. 

Troponin I Expression in Human ES-derived Cardiomyocytes. 

Troponin I Expression in Human ES-derived Cardiomyocytes.BG01V human embryonic stem cells were differentiated into cardiomyocytes using the StemXVivo®Cardiomyocyte Differentiation Kit (Catalog # SC032B) and maintained in StemXVivo®Cardiomyocyte Maintenance Media Supplement (Catalog # AR011). Troponin I (red) expression was detected in cardiomyocytes at day 80 of differentiation. The nuclei were counterstained with DAPI (blue).

Formulation, Preparation, and Storage

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: Embryonic Stem Cells

Embryonic stem (ES) cells are pluripotent stem cells derived from the inner cell mass of pre-implantation embryos. Induced pluripotent stem (iPS) cells can be generated by somatic cell reprogramming following the exogenous expression of specific transcription factors (Oct-3/4, KLF4, SOX2, and c-Myc). These cell types are capable of unlimited, undifferentiated proliferation in vitro and still maintain the capacity to differentiate into a wide variety of somatic cells. In this capacity, pluripotent stem cells have widespread clinical potential for the treatments of heart disease, diabetes, spinal cord injury, and a variety of neurodegenerative disorders.

R&D Systems offers a wide range of products to support pluripotent stem cell culture and differentiation. Mouse embryonic fibroblasts may be used to maintain and expand pluripotent stem cells in an undifferentiated state. We also offer defined culture media, which are specifically optimized for use with human or rodent pluripotent stem cells. In addition, R&D Systems offers a variety of products to assess differentiation status and identify specific stem cell types of interest, including panels of marker antibodies, primer pairs, multi-color flow cytometry kits, and specialized verification kits.

Additional Embryonic Stem Cells Products

Product Documents for StemXVivo Cardiomyocyte Maintenance Media Supplement (50X)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for StemXVivo Cardiomyocyte Maintenance Media Supplement (50X)

For research use only

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Protocols

View specific protocols for StemXVivo Cardiomyocyte Maintenance Media Supplement (50X) (AR011):

Refer to the product datasheet for complete product details.

Briefly, Cardiomyocyte Maintenance Media is prepared using the following procedure:

  • Dilute 50-fold in RPMI 1640
  • Add 0.5 mM L-glutamine and 1X Penicillin / Streptomycin (optional)
  • Store the media in the dark at 2-8 °C
  • Use within 2 weeks
 

 

Reagents Provided

Reagents provided in the N21-MAX Insulin Free Media Supplement (Catalog # AR010):

  • RPMI 1640
  • L-Glutamine
  • Penicillin/Streptomycin

 

Other Supplies Required

Reagents

  • Basal media (e.g., Neurobasal Media from Invitrogen®, Catalog # 21103-029 or equivalent)

Materials

  • Serological pipettes
  • Pipettes and pipette tips

Equipment

  • 2 °C to 8 °C refrigerator

 

Procedure Overview

Dilute 50-fold in RPMI 1640. Add 0.5 mM L-glutamine and 1X Penicillin / Streptomycin (optional). Store the media in the dark at 2 to 8 °C for up to two weeks.

FAQs for StemXVivo Cardiomyocyte Maintenance Media Supplement (50X)

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  • Q: How many days can cardiomyocytes be maintained using StemXVivo® Cardiomyocyte Maintenance Media Supplement (Catalog # AR011)?

    A:

    Stem cell-derived cardiomyocytes can be maintained using StemXVivo® Cardiomyocyte Maintenance Media Supplement (Catalog # AR011) for longer than 200 days. You will notice that the cells will become less adherent to the plate over time, but will continue to beat and express proper cardiac markers.

  • Q: When using the StemXVivo® Cardiomyocyte Differentiation Kit, when should the switch be made to the StemXVivo® Cardiomyocyte Maintanence Media Supplement (Catalog # AR011)?

    A: The switch to maintenance media can be made any time after beating is initiated or upon the completion of the reagents provided in the kit.

  • Q: How many days can cardiomyocytes be maintained using StemXVivo® Cardiomyocyte Maintenance Media Supplement (Catalog # AR011)?

    A:

    Stem cell-derived cardiomyocytes can be maintained using StemXVivo® Cardiomyocyte Maintenance Media Supplement (Catalog # AR011) for longer than 200 days. You will notice that the cells will become less adherent to the plate over time, but will continue to beat and express proper cardiac markers.

  • Q: When using the StemXVivo® Cardiomyocyte Differentiation Kit, when should the switch be made to the StemXVivo® Cardiomyocyte Maintanence Media Supplement (Catalog # AR011)?

    A: The switch to maintenance media can be made any time after beating is initiated or upon the completion of the reagents provided in the kit.

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