Human Troponin T (Cardiac) Antibody

Catalog # Availability Size / Price Qty
MAB1874
MAB1874-SP
Human Troponin T (Cardiac) Antibody in Immunocytochemistry
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Product Details
Citations (5)
FAQs
Supplemental Products
Reviews (3)

Human Troponin T (Cardiac) Antibody Summary

Species Reactivity
Human
Specificity
Detects purified human Troponin T (Cardiac) in direct ELISAs.
Source
Monoclonal Mouse IgG2A Clone # 200805
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human cardiac Troponin T
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunocytochemistry Troponin T in BG01V Human Embryonic Stem Cells. View Larger

Troponin T in BG01V Human Embryonic Stem Cells. Troponin T was detected in immersion fixed BG01V human embryonic stem cells differentiated into cardiomyocytes using Mouse Anti-Human Troponin T (Cardiac) Monoclonal Antibody (Catalog # MAB1874) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Immunohistochemistry Troponin T in Human Heart. View Larger

Troponin T in Human Heart. Troponin T was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human Troponin T (Cardiac) Monoclonal Antibody (Catalog # MAB1874) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cardiomyocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Troponin T

Troponin T is one of three troponin isoforms found in the tropomyosin-troponin complex. This complex is responsible for the calcium sensitivity of the contractile apparatus in the muscle. Cardiac Troponin T is used as a biological marker for cardiomyocytes and its level in serum is frequently used as an indicator of myocardial cell injury.

Entrez Gene IDs
7139 (Human)
Alternate Names
Cardiac Muscle Troponin T; Cardiomyopathy, Dilated 1D (Autosomal Dominant); Cardiomyopathy, Hypertrophic; CMD1D; CMH2; CMPD2; cTnT; LVNC6; RCM3; TNNT2; TnTC; troponin T type 2 (cardiac); Troponin T; troponin T, cardiac muscle; Troponin T2, Cardiac Type; Truncated Cardiac Troponin T

Product Datasheets

Citations for Human Troponin T (Cardiac) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Chemical-defined and albumin-free generation of human atrial and ventricular myocytes from human pluripotent stem cells
    Authors: F Pei, J Jiang, S Bai, H Cao, L Tian, Y Zhao, C Yang, H Dong, Y Ma
    Stem Cell Res, 2017;19(0):94-103.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Differentiation and characterization of rhesus monkey atrial and ventricular cardiomyocytes from induced pluripotent stem cells
    Authors: X Zhang, H Cao, S Bai, W Huo, Y Ma
    Stem Cell Res, 2017;20(0):21-29.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  3. Direct differentiation of atrial and ventricular myocytes from human embryonic stem cells by alternating retinoid signals.
    Authors: Zhang Q, Jiang J, Han P, Yuan Q, Zhang J, Zhang X, Xu Y, Cao H, Meng Q, Chen L, Tian T, Wang X, Li P, Hescheler J, Ji G, Ma Y
    Cell Res., 2011;21(4):579-87.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Expansion of human cardiac stem cells in physiological oxygen improves cell production efficiency and potency for myocardial repair.
    Authors: Li TS, Cheng K, Malliaras K, Matsushita N, Sun B, Marban L, Zhang Y, Marban E
    Cardiovasc. Res., 2011;89(1):157-65.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. Stem cells derived from human fetal membranes display multilineage differentiation potential.
    Authors: Ilancheran S, Michalska A, Peh G, Wallace EM, Pera M, Manuelpillai U
    Biol. Reprod., 2007;77(3):577-88.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Troponin T (Cardiac) Antibody

Average Rating: 5 (Based on 3 Reviews)

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Human Troponin T (Cardiac) Antibody
By Anonymous on 07/04/2018
Application: Flow Sample Tested: iPSC-CMs Species: Human

Human Troponin T (Cardiac) Antibody
By Anonymous on 05/19/2018
Application: ICC/IF Sample Tested: Human iPSC-Derived Cardiomyocytes Species: Human

Human Troponin T (Cardiac) Antibody
By Anonymous on 12/15/2016
Application: ICC/IF Sample Tested: Induced Pluripotent Stem Cells Species: Human

We use this 'Human Troponin T (Cardiac) Antibody' for staining differentiated cardiac cells from non-muscle cells (iPSc, Fibroblasts, etc.).