STEP Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-03693

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all STEP Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 190-307 of human STEP (NP_116170.3). SFTYSEWMEEKIEDDFLDLDPVPETPVFDCVMDIKPEADPTSLTVKSMGLQERRGSNVSLTLDMCTPGCNEEGFGYLMSPREESAREYLLSASRVLQAEELHEKALDPFLLQAEFFEI

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

64 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for STEP Antibody - Azide and BSA Free

Western Blot: STEP AntibodyAzide and BSA Free [NBP3-03693]

Western Blot: STEP AntibodyAzide and BSA Free [NBP3-03693]

Western Blot: STEP Antibody [NBP3-03693] - Western blot analysis of extracts of various cell lines, using STEP antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
STEP Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: STEP Antibody - Azide and BSA Free [NBP3-03693] -

Immunocytochemistry/ Immunofluorescence: STEP Antibody - Azide and BSA Free [NBP3-03693] - Immunofluorescence analysis of Neuro-2a cells using STEP Rabbit pAb (A16585) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
STEP Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: STEP Antibody - Azide and BSA Free [NBP3-03693] -

Immunocytochemistry/ Immunofluorescence: STEP Antibody - Azide and BSA Free [NBP3-03693] - Immunofluorescence analysis of SH-SY5Y cells using STEP Rabbit pAb (A16585) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Applications for STEP Antibody - Azide and BSA Free

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: STEP

The protein tyrosine phosphatase non-receptor type 5 or striatum-enriched protein-tyrosine phosphatase (STEP) protein is part of a family of protein tyrosine phosphatases enriched within the CNS, and is a key component of the NMDAR complex. It is expressed within dopaminoceptive neurons in the CNS and particularly enriched within the basal ganglia and related structures. STEP61 is the membrane-associated isoform and STEP46 is the cytosolic protein. In response to transient hypoxia-ischemia in the brain, STEP61 is proteolytically cleaved by calpain, thereby releasing a smaller isoform, STEP33.

Long Name

Tyrosine-protein phosphatase non-receptor type 5

Alternate Names

PTPN5

Gene Symbol

PTPN5

Additional STEP Products

Product Documents for STEP Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for STEP Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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