STK38 Antibody (2F3) - Azide and BSA Free

Novus Biologicals | Catalog # H00011329-M04

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2F3

Format

Azide and BSA Free
View all NDR1/STK38 Primary Antibodies »

Product Specifications

Immunogen

STK38 (AAH12085, 365 a.a. ~ 465 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EHRIGAPGVEEIKSNSFFEGVDWEHIRERPAAISIEIKSIDDTSNFDEFPESDILKPTVATSNHPETDYKNKDWVFINYTYKRFEGLTARGAIPSYMKAAK

Localization

Cytoplasmic and Nuclear

Specificity

STK38 - serine/threonine kinase 38

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for STK38 Antibody (2F3) - Azide and BSA Free

Western Blot: STK38 Antibody (2F3) [H00011329-M04]

Western Blot: STK38 Antibody (2F3) [H00011329-M04]

Western Blot: STK38 Antibody (2F3) [H00011329-M04] - Analysis of STK38 expression in NIH/3T3 (Cat # L018V1).
Western Blot: STK38 Antibody (2F3) [H00011329-M04]

Western Blot: STK38 Antibody (2F3) [H00011329-M04]

Western Blot: STK38 Antibody (2F3) [H00011329-M04] - Western blot analysis of STK38 over-expressed 293 cell line, cotransfected with STK38 Validated Chimera RNAi or non-transfected control. Blot probed with H00011329-M04. GAPDH (36.1 kDa) used as loading control.
Western Blot: STK38 Antibody (2F3) [H00011329-M04]

Western Blot: STK38 Antibody (2F3) [H00011329-M04]

Western Blot: STK38 Antibody (2F3) [H00011329-M04] - Analysis of STK38 expression in PC-12 (Cat # L012V1).
Western Blot: STK38 Antibody (2F3) [H00011329-M04]

Western Blot: STK38 Antibody (2F3) [H00011329-M04]

Western Blot: STK38 Antibody (2F3) [H00011329-M04] - Analysis of STK38 expression in Hela S3 NE (Cat # L013V3).
Western Blot: STK38 Antibody (2F3) [H00011329-M04]

Western Blot: STK38 Antibody (2F3) [H00011329-M04]

Western Blot: STK38 Antibody (2F3) [H00011329-M04] - Analysis of STK38 expression in Raw 264.7 (Cat # L024V1).
Western Blot: STK38 Antibody (2F3) [H00011329-M04]

Western Blot: STK38 Antibody (2F3) [H00011329-M04]

Western Blot: STK38 Antibody (2F3) [H00011329-M04] - Analysis of STK38 expression in transfected 293T cell line by STK38 monoclonal antibody (M04), clone 2F3. Lane 1: STK38 transfected lysatE (54.2 KDa). Lane 2: Non-transfected lysate.
ELISA: STK38 Antibody (2F3) [H00011329-M04]

ELISA: STK38 Antibody (2F3) [H00011329-M04]

ELISA: STK38 Antibody (2F3) [H00011329-M04] - Detection limit for recombinant GST tagged STK38 is approximately 0.03ng/ml as a capture antibody.

Applications for STK38 Antibody (2F3) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for RNAi validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NDR1/STK38

STK38 belongs to the NDR family of serine/threonine protein kinases. NDR kinases require the phosphorylation of conserved Ser/Thr residues for activation. NDR family members have two unique stretches of primary sequence: an N-terminal regulatory (NTR) domain and an insert of several residues between subdomains VII and VIII of the kinase domain. The kinase domain insert functions as an auto-inhibitory sequence (AIS), while binding of the co-activator MOB (Mps-one binder) proteins to the NTR domain releases NDR kinases from inhibition of autophosphorylation. STK38 negatively regulates the activation of MEKK1/2 by direct interaction with the catalytic domain of MEKK1/2. The negative regulation of MEKK1/2 is not due to its phosphorylation by STK38.

Long Name

Nuclear Dbf2-related Kinase 1

Alternate Names

STK38

Entrez Gene IDs

11329 (Human)

Gene Symbol

STK38

OMIM

606964 (Human)

UniProt

AAH12085 (Human)

Additional NDR1/STK38 Products

Product Documents for STK38 Antibody (2F3) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for STK38 Antibody (2F3) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for STK38 Antibody (2F3) - Azide and BSA Free

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Protocols

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FAQs

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