Recombinant Streptolysin Protein

Novus Biologicals | Catalog # NBP2-61445

Novus Biologicals
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Key Product Details

Source

E. coli
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Product Specifications

Description

A single non-glycosylated polypeptide chain containing 538 amino acids corresponding to Streptolysin Source: E. coli

Uniprot ID: P0DF97

Amino Acid Sequence: NKQNTASTET TTTNEQPKPE SSELTTEKAG QKTDDMLNSN DMIKLAPKEM PLESAEKEEK KSEDKKKSEE DHTEEINDKI YSLNYNELEV LAKNGETIEN FVPKEGVKKA DKFIVIERKK KNINTTPVDI SIIDSVTDRT YPAALQLANK GFTENKPDAV VTKRNPQKIH IDLPGMGDKA TVEVNDPTYA NVSTAIDNLV NQWHDNYSGG NTLPARTQYT ESMVYSKSQI EAALNVNSKI LDGTLGIDFK SISKGEKKVM IAAYKQIFYT VSANLPNNPA DVFDKSVTFK ELQRKGVSNE APPLFVSNVA YGRTVFVKLE TSSKSNDVEA AFSAALKGTD VKTNGKYSDI LENSSFTAVV LGGDAAEHNK VVTKDFDVIR NVIKDNATFS RKNPAYPISY TSVFLKNNKI AGVNNRTEYV ETTSTEYTSG KINLSHQGAY VAQYEILWDE INYDDKGKEV ITKRRWDNNW YSKTSPFSTV IPLGANSRNI RIMARECTGL AWEWWRKVID ERDVKLSKEI NVNISGSTLS PYGSITYK

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of Streptolysin as determined by LAL method.

Predicted Molecular Mass

60.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Data Not Available.

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation, and Storage

NBP2-61445
Formulation Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative No Preservative
Concentration Lyoph
Reconstitution Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Streptolysin

Bacteria of the Streptococcus genera produce the exotoxin streptolysin. Anti-streptolysin antibody titers in patients are measured as a marker of inflammation. Streptolysin is also an important tool in cell biology due to its hemolytic activity and the ability to permeabilize plasma membranes.

Alternate Names

Slo, Thiol activated cytolysin

Additional Streptolysin Products

Product Documents for Recombinant Streptolysin Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Streptolysin Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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