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Substrate Reagent Pack

R&D Systems | Catalog # DY999B

New! 16 Vials TMB ELISA Substrate
R&D Systems
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Key Product Details

Applications

ELISA

Conjugate

NL637

Product Summary for Substrate Reagent Pack

INTENDED USE & DESCRIPTION

Substrate Reagent Pack contains 16 vials of TMB ELISA Substrate producing color development when contacting HRP-conjugated or biotinylated, streptavidin-HRP bound detection antibodies.   

STORAGE & STABILITY

Store unopened at 2-8 °C. Stable for up to 30 days after opening when stored at 2-8 °C.

REAGENT PREPARATION

Warm to room temperature before use. Protect from light. Add 100 µL to each well and incubate at room temperature for 20 minutes while protecting from light. Add Stop Solution (Catalog # DY994B or DY994B-250) and read the microplate.

 

Formulation, Preparation, and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Product Documents for Substrate Reagent Pack

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Substrate Reagent Pack

For research use only

Citations for Substrate Reagent Pack

Customer Reviews for Substrate Reagent Pack (1)

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  • Substrate Reagent Pack
    Name: Maximiliaan Hennink
    Verified Customer | Posted 08/06/2025
    Worked great for my antibody ELISA. Easy to use, signal came fast (stopped the reaction after 10 minutes)
    Substrate Reagent Pack DY999B

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FAQs for Substrate Reagent Pack

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    • A: Yes, TMB ELISA Substrate (Catalog # DY999B) has been tested for equivalency and can be substituted for a 1:1 mixture of Color Reagent A and B (Catalog # DY999).
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