SUMO Activating Enzyme E1 (SAE1) Antibody (0L2N2)

Novus Biologicals | Catalog # NBP3-15317

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 0L2N2 expressed in HEK293
View all SUMO Activating Enzyme E1 (SAE1/UBA2) Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 200-300 of human SUMO Activating Enzyme E1 (SAE1) (Q9UBE0). DSSETTMVKKKVVFCPVKEALEVDWSSEKAKAALKRTTSDYFLLQVLLKFRTDKGRDPSSDTYEEDSELLLQIRNDVLDSLGISPDLLPEDFVRYCFSEMA

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SUMO Activating Enzyme E1 (SAE1) Antibody (0L2N2)

Western Blot: SUMO Activating Enzyme E1 (SAE1) Antibody (0L2N2) [NBP3-15317]

Western Blot: SUMO Activating Enzyme E1 (SAE1) Antibody (0L2N2) [NBP3-15317]

Western Blot: SUMO Activating Enzyme E1 (SAE1) Antibody (0L2N2) [NBP3-15317] - Western blot analysis of extracts of various cell lines, using SUMO Activating Enzyme E1 (SAE1) Rabbit mAb (NBP3-15317) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Western Blot: SUMO Activating Enzyme E1 (SAE1) Antibody (0L2N2) [NBP3-15317]

Western Blot: SUMO Activating Enzyme E1 (SAE1) Antibody (0L2N2) [NBP3-15317]

Western Blot: SUMO Activating Enzyme E1 (SAE1) Antibody (0L2N2) [NBP3-15317] - Western blot analysis of extracts of various cell lines, using SUMO Activating Enzyme E1 (SAE1) Rabbit mAb (NBP3-15317) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Applications for SUMO Activating Enzyme E1 (SAE1) Antibody (0L2N2)

Application
Recommended Usage

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SUMO Activating Enzyme E1 (SAE1/UBA2)

The dimeric enzyme acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins and formation of a thioester with a conserved cysteine residue on SAE2

Long Name

SUMO1 Activating Enzyme E1

Alternate Names

AOS1, SAE1, SUA1, UBA2, UBLE1A

Gene Symbol

SAE1

Additional SUMO Activating Enzyme E1 (SAE1/UBA2) Products

Product Documents for SUMO Activating Enzyme E1 (SAE1) Antibody (0L2N2)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for SUMO Activating Enzyme E1 (SAE1) Antibody (0L2N2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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