Canine Surfactant Protein A ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP3-42465

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, Plasma, Lung Lavage Fluid, Tissue homogenates and other biological fluids (100 uL)

Sensitivity

2.73 pg/mL (example only; lot dependent)

Assay Range

6.25 - 400 pg/mL (example only; lot dependent)
View all SFTPA1 ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Canine

Description

Assay time: 3 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV 12 (example only; lot dependent)

Recovery for Canine Surfactant Protein A ELISA Kit (Colorimetric)

Recovery

84 - 98% (example only; lot dependent)

Kit Contents for Canine Surfactant Protein A ELISA Kit (Colorimetric)

  • Assay Diluent A
  • Assay Diluent B
  • Detection Reagent A
  • Detection Reagent B
  • Instruction manual
  • Plate sealer for 96 wells
  • Pre-coated ready to use 96-well strip plate
  • Standard
  • Standard Diluent
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: SFTPA1

Surfactant protein A is a protein with two isoforms, with lengths of 248 and 263 amino acids and weights of approximately 26 and 28 kDa respectively. Surfactant protein A binds to surfactant phospholipids in the presence of calcium ions and helps lower the surface tension in the alveoli of the lungs. Current research is being done on several diseases and disorders linked to this protein including pulmonary fibrosis, allergic bronchopulmonary aspergillosis, pulmonary alveolar proteinosis, non-small cell lung carcinoma, sclerosing hemangioma, otitis media, chronic obstructive pulmonary disease, bronchitis obliterans, bronchopulmonary dysplasia, rhinitis, aspergillosis, pulmonary edema, insulin resistance, and pneumoconiosis. Surfactant protein A has also been shown to have interactions with DMBT1, MYO18A, C1QA, SFTPD, and TLR2 in pathways such as the immune response, bacterial infections in CF airways, cell-cell communication, phagosome, and pertussis pathways.

Long Name

Surfactant Protein A1

Alternate Names

COLEC4, PSP-A, SP-A, SP-A1

Gene Symbol

SFTPA1

Additional SFTPA1 Products

Product Documents for Canine Surfactant Protein A ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Canine Surfactant Protein A ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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