Synaptotagmin 1 [p Ser309] Antibody - Azide Free

Novus Biologicals | Catalog # NB300-236

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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Rat

Predicted:

Bovine (100%), Canine (100%), Chicken (100%), Human (100%), Mouse (100%), Primate (100%), Zebrafish (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide Free
View all Synaptotagmin-1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser309 conjugated to KLH. Accession # P21707

Modification

p Ser309

Localization

Synaptic vesicles and chromaffin granules.

Specificity

Specific for endogenous levels of the ~62 kDa synaptotagmin 1 protein phosphorylated at Ser 309. Immunolabeling is completely eliminated by treatment with lambda-phosphatase.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Synaptotagmin 1 [p Ser309] Antibody - Azide Free

Western Blot: Synaptotagmin 1 [p Ser309] Antibody [NB300-236]

Western Blot: Synaptotagmin 1 [p Ser309] Antibody [NB300-236]

Western Blot: Synaptotagmin 1 [p Ser309] Antibody [NB300-236] - Western blot of rat cortex lysate showing specific immunolabeling of the ~60k - ~62k synaptotagmin phosphorylated at Ser309 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated (1200 units for 30 min) before being exposed to the Ser309 synaptotagmin antibody. The immunolabeling is completely eliminated by treatment with lambda-phosphatase.
Immunohistochemistry: Synaptotagmin 1 [p Ser309] Antibody [NB300-236]

Immunohistochemistry: Synaptotagmin 1 [p Ser309] Antibody [NB300-236]

Immunohistochemistry: Synaptotagmin-1 [p Ser309] Antibody [NB300-236] - Immunostaining of 14 DIV rat cortical neurons showing synaptotagmin when phosphorylated at Ser309 in red and PSD95 in green. Photo courtesy of Gang Liu.

Applications for Synaptotagmin 1 [p Ser309] Antibody - Azide Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:400

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Format

Azide Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Synaptotagmin-1

Synaptotagmin is widely regarded as the primary calcium sensor for synaptic vesicle exocytosis (Fernandez-Chacon et al., 2001; Wang et al., 2003). Moreover recent studies indicate that the protein also plays a key role in endocytosis (Poskanzer et al., 2003). Synaptotagmin can be phosphorylated by multiple protein kinases and this may play a key role in modulation of synaptotagmin ability to influence both the exocytotic and endocytotic components of synaptic transmission (Hilfiker et al., 1999; Lee et al., 2004).

Alternate Names

P65, SVP65, Synaptotagmin1, SYT1

Entrez Gene IDs

25716 (Rat)

Gene Symbol

SYT1

UniProt

P21707 (Rat)

Additional Synaptotagmin-1 Products

Product Documents for Synaptotagmin 1 [p Ser309] Antibody - Azide Free

Certificate of Analysis

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Product Specific Notices for Synaptotagmin 1 [p Ser309] Antibody - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Synaptotagmin 1 [p Ser309] Antibody - Azide Free

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Protocols

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FAQs

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