Synaptotagmin 4 Antibody (5F8) - Azide and BSA Free

Novus Biologicals | Catalog # H00006860-M04

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Kappa Clone # 5F8

Format

Azide and BSA Free
View all Synaptotagmin 4 Primary Antibodies »

Product Specifications

Immunogen

SYT4 (NP_065834, 1 a.a. ~ 99 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAPITTSREEFDEIPTVVGIFSAFGLVFTVSLFAWICCQRKSSKSNKTPPYKFVHVLKGVDIYPENLNSKKKFGADDKNEVKNKPAVPKNSLHLDLEKR

Specificity

SYT4 (5F8)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3 Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Synaptotagmin 4 Antibody (5F8) - Azide and BSA Free

Western Blot: Synaptotagmin 4 Antibody (5F8) [H00006860-M04]

Western Blot: Synaptotagmin 4 Antibody (5F8) [H00006860-M04]

Western Blot: Synaptotagmin 4 Antibody (5F8) [H00006860-M04] - Analysis of SYT4 expression in A-431 (Cat # L015V1).
Immunocytochemistry/ Immunofluorescence: Synaptotagmin 4 Antibody (5F8) [H00006860-M04]

Immunocytochemistry/ Immunofluorescence: Synaptotagmin 4 Antibody (5F8) [H00006860-M04]

Immunocytochemistry/Immunofluorescence: Synaptotagmin 4 Antibody (5F8) [H00006860-M04] - Analysis of monoclonal antibody to SYT4 on A-431 cell. Antibody concentration 10 ug/ml
ELISA: Synaptotagmin 4 Antibody (5F8) [H00006860-M04]

ELISA: Synaptotagmin 4 Antibody (5F8) [H00006860-M04]

ELISA: Synaptotagmin 4 Antibody (5F8) [H00006860-M04] - Detection limit for recombinant GST tagged SYT4 is approximately 0.03ng/ml as a capture antibody.

Applications for Synaptotagmin 4 Antibody (5F8) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Synaptotagmin 4

Synaptotagmin 4 may be involved in Ca(2+)-dependent exocytosis of secretory vesicles through Ca(2+) and phospholipid binding to the C2 domain or may serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis

Alternate Names

HsT1192, KIAA1342sytIV, synaptotagmin 4, synaptotagmin IVsynaptotagmin-4, SytIV

Entrez Gene IDs

6860 (Human)

Gene Symbol

SYT4

OMIM

600103 (Human)

UniProt

NP_065834 (Human)

Additional Synaptotagmin 4 Products

Product Documents for Synaptotagmin 4 Antibody (5F8) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Synaptotagmin 4 Antibody (5F8) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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