SynGAP Antibody

Novus Biologicals | Catalog # NB120-3344

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all SynGAP Primary Antibodies »

Product Specifications

Immunogen

GST fusion protein of a recombinant fragment, residues 947-1167, of rat SynGAP.

Reactivity Notes

Cross-reacts with Rat and Mouse.Not yet tested in other species.

Specificity

This detects synaptic Ras-GTPase activating protein (SynGAP) from mouse and rat tissues.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SynGAP Antibody

Immunocytochemistry/ Immunofluorescence: SynGAP Antibody [NB120-3344]

Immunocytochemistry/ Immunofluorescence: SynGAP Antibody [NB120-3344]

Immunocytochemistry/Immunofluorescence: SynGAP Antibody [NB120-3344] - Immunofluorescence analysis of SynGAP was performed using 70% confluent log phase PC-12 cells.
Immunohistochemistry-Paraffin: SynGAP Antibody [NB120-3344]

Immunohistochemistry-Paraffin: SynGAP Antibody [NB120-3344]

Immunohistochemistry-Paraffin: SynGAP Antibody [NB120-3344] - Immunohistochemistry analysis of SYNGAP showing staining in the cytoplasm of paraffin-embedded human heart tissue (right) compared to a negative control without primary antibody (left).
Immunocytochemistry/ Immunofluorescence: SynGAP Antibody [NB120-3344]

Immunocytochemistry/ Immunofluorescence: SynGAP Antibody [NB120-3344]

Immunocytochemistry/Immunofluorescence: SynGAP Antibody [NB120-3344] - Rat hippocampal neurons
Immunocytochemistry/ Immunofluorescence: SynGAP Antibody [NB120-3344]

Immunocytochemistry/ Immunofluorescence: SynGAP Antibody [NB120-3344]

Immunocytochemistry/Immunofluorescence: SynGAP Antibody [NB120-3344]

Applications for SynGAP Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1 ug/mL

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
Immunocytochemistry: use at a concentration of 1 ug/ml.Western Blot: use at a concentration of 1 ug/ml.Not tested in other applications.Optimal dilutions / concentrations should be determined by the end user.By Western blot, this antibody detects an ~135 kDa protein representing SynGAP from mouse brain tissue homogenate. Immunocytochemical staining of SynGAP in rat hippocampal neurons yields a pattern consistent with vesicular staining.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS with 1 mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SynGAP

Synaptic Ras GTPase Activating Protein (SynGAP) is a PDZ-domain interacting protein expressed almost exclusively in the brain. SynGAP has been shown to be a major component of postsynaptic density and associates with post synaptic density 95 kDa (PSD-95), a synaptic scaffold protein, and synaptic N-methyl-D-aspartate (NMDA) receptors. SynGAP Ras-GTPase activity has also been shown to be inhibited by phosphorylation by CaM kinase II, another abundant protein located in the postsynaptic density. It has been proposed that SynGAP may function as a negative regulator of MAP Kinase activation in the absence of active NMDA receptors.

Alternate Names

DKFZp761G1421, KIAA1938135kDa, MRD5, synaptic Ras GTPase activating protein 1, synaptic Ras GTPase activating protein 1 homolog (rat), Synaptic Ras-GAP 1

Entrez Gene IDs

240057 (Mouse); 192117 (Rat)

Gene Symbol

SYNGAP1

Additional SynGAP Products

Product Documents for SynGAP Antibody

Certificate of Analysis

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Product Specific Notices for SynGAP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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