Syntaxin 16 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-92467

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (98%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Syntaxin 16 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SPLHSRSIAAELDELADDRMALVSGISLDPEAAIGVTKRPPPKWVDGVDEIQYDVGRIKQKMKELASLHDKHLNRPTLDDSSEEEHAIEITTQEITQLFHR

Reactivity Notes

Rat (87%).

Marker

Trans-Golgi Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit Syntaxin 16 Antibody - BSA Free (NBP1-92467) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and Simple Western. Anti-Syntaxin 16 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Syntaxin 16 Antibody - BSA Free

Western Blot: Syntaxin 16 Antibody [NBP1-92467]

Western Blot: Syntaxin 16 Antibody [NBP1-92467]

Western Blot: Syntaxin 16 Antibody [NBP1-92467] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: Human cell line MCF-7
Immunocytochemistry/ Immunofluorescence: Syntaxin 16 Antibody [NBP1-92467]

Immunocytochemistry/ Immunofluorescence: Syntaxin 16 Antibody [NBP1-92467]

Immunocytochemistry/Immunofluorescence: Syntaxin 16 Antibody [NBP1-92467] - Staining of human cell line A-431 shows localization to vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Syntaxin 16 Antibody [NBP1-92467]

Immunohistochemistry-Paraffin: Syntaxin 16 Antibody [NBP1-92467]

Immunohistochemistry-Paraffin: Syntaxin 16 Antibody [NBP1-92467] - Staining of human lateral ventricle shows strong cytoplasmic positivity in neuronal cells.
Simple Western: Syntaxin 16 Antibody [NBP1-92467]

Simple Western: Syntaxin 16 Antibody [NBP1-92467]

Simple Western: Syntaxin 16 Antibody [NBP1-92467] - Simple Western lane view shows a specific band for Syntaxin 16 in 0.2 mg/ml of MCF-7 lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation systems.
Simple Western: Syntaxin 16 Antibody [NBP1-92467]

Simple Western: Syntaxin 16 Antibody [NBP1-92467]

Simple Western: Syntaxin 16 Antibody [NBP1-92467] - Electropherogram image of the corresponding Simple Western lane view. Syntaxin 16 antibody was used at 1:100 dilution on MCF-7 lysate(s) respectively.
Syntaxin 16 Antibody - BSA Free Western Blot: Syntaxin 16 Antibody - BSA Free [NBP1-92467]

Western Blot: Syntaxin 16 Antibody - BSA Free [NBP1-92467]

Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: Human cell line MCF-7

Applications for Syntaxin 16 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Simple Western

1:100

Western Blot

0.04-0.4 ug/ml
Application Notes

For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
See Simple Western Antibody Database for Simple Western validation: Tested in MCF-7 lysates, separated by Size, antibody dilution of 1:100

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Syntaxin 16

Syntaxin 16 is a SNARE involved in a vesicular transport step within the Golgi stack. It is ubiquitously expressed in Drosophila and localizes to the Golgi apparatus.

Alternate Names

STX16, Syn16

Gene Symbol

STX16

Additional Syntaxin 16 Products

Product Documents for Syntaxin 16 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Syntaxin 16 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Syntaxin 16 Antibody - BSA Free

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Protocols

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