TAS2R4 Antibody

Novus Biologicals | Catalog # NB110-74890

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Primate

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all TAS2R4 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide from human TAS2R4 conjugated to blue carrier protein was used as the antigen.

Reactivity Notes

Marmoset

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for TAS2R4 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:1000

Immunohistochemistry-Frozen

1:1000

Immunohistochemistry-Paraffin

1:1000

Western Blot

1:1000
Application Notes
Useful in Immunofluorescence (PMID: 21303656).

Formulation, Preparation, and Storage

Purification

Unpurified

Reconstitution

Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size is provided in reconstituted format.

Formulation

Lyophilized from whole antisera

Preservative

No Preservative

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TAS2R4

This gene encodes a member of a family of candidate taste receptors that are members of the G protein-coupled receptor superfamily and that are specifically expressed by taste receptor cells of the tongue and palate epithelia. These apparently intronless genes encode a 7-transmembrane receptor protein, functioning as a bitter taste receptor. This gene is clustered with another 3 candidate taste receptor genes in chromosome 7 and is genetically linked to loci that influence bitter perception. FUNCTION: Gustducin-coupled receptor for denatonium and N(6)-propyl-2-thiouracil implicated in the perception of bitter compounds in the oral cavity and the gastrointestinal tract. Signals through PLCB2 and the calcium-regulated cation channel TRPM5. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Expressed in subsets of taste receptor cells of the tongue and palate epithelium and exclusively in gustducin-positive cells. MISCELLANEOUS: Several bitter taste receptors are expressed in a single taste receptor cell. Useful Links; The Human Olfactory Data Exploratorium: HORDE; Olfactory Receptor Data Base: ORDB; Transmembrane Analysis: TM analysis

Alternate Names

candidate taste receptor T2R4, taste receptor, type 2, member 4

Entrez Gene IDs

50832 (Human)

Gene Symbol

TAS2R4

UniProt

Q9NYW5 (Human)

Additional TAS2R4 Products

Product Documents for TAS2R4 Antibody

Certificate of Analysis

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Download SDS

Product Specific Notices for TAS2R4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TAS2R4 Antibody

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Protocols

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FAQs

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