![Western Blot: Tau Antibody [NBP3-11044]](https://resources.rndsystems.com/images/products/Tau-Antibody-Western-Blot-NBP3-11044-img0001.jpg "Western Blot: Tau Antibody [NBP3-11044]")
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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, Dot Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit
Format
BSA Free
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Product Specifications
Immunogen
Recombinant Human Tau AA297-391 (dGAE) Fibril (NP_005901.2)
Localization
Cytoplasm, Axolemma, Axolemma Plasma Membrane, Axon, Cell Body, Cell membrane, Cytoplasmic Ribonucleoprotein Granule, Cytoplasmic Side, Cytoskeleton, Cytosol, Dendrite, Growth cone, Microtubule, Microtubule Associated Complex, Neurofibrillary Tangle, Neuronal Cell Body, Nuclear Periphery, Nuclear Speck, Nucleus, Peripheral membrane protein, Plasma Membrane, Tubulin Complex
Specificity
Detects ~10.2 kDa. Detects other forms of Tau.
Clonality
Polyclonal
Host
Rabbit
Scientific Data Images for Tau Antibody - BSA Free
Western Blot: Tau Antibody [NBP3-11044]
Western Blot: Tau Antibody [NBP3-11044] - Western blot analysis of Mouse, Rat Brain showing detection of Multiple bands due to different isoforms/aggregation states Tau protein using Rabbit Anti-Tau Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Mouse brain (15 ug). Lane 2: Rat brain (15 ug). Load: 15 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-Tau Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: Multiple bands due to different isoforms/aggregation states. Other Band(s): 50 kDa, 65 kDa, 100 kDa.
Dot Blot: Tau Antibody [NBP3-11044] - Dot blot analysis using Rabbit Anti-Tau Polyclonal Antibody. Tissue: Recombinant Tau Monomers and Fibrils. Species: Human. Primary Antibody: Rabbit Anti-Tau Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1 hour at RT with shaking.
Applications for Tau Antibody - BSA Free
Application
Recommended Usage
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.4), 50% Glycerol
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: Tau
Long Name
Microtubule-Associated Protein Tau
Alternate Names
MAPT, MSTD, MTBT1, Neurofibrillary tangle protein, PPND
Gene Symbol
MAPT
Additional Tau Products
Product Documents for Tau Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Tau Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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