TBI

Tocris Bioscience | Catalog # 7660

Fluorogenic ligand for Broccoli RNA aptamer
Tocris Bioscience

Key Product Details

Description

Fluorogenic ligand for Broccoli RNA aptamer

Wavelength

Excitation = 485 nm, Emission = 527 nm
View all Light-up Aptamers »

Product Description

TBI is a DFHBI derivative for imaging of single Broccoli-tagged mRNA in cells. Excitation and emission maxima (λ) when bound to Broccoli are 485 and 527 nm, respectively. TBI shows higher affinity for Broccoli aptamers compared with DFHBI-1T (Cat. No. 5610) (KD values are 71 and 305 nM, respectively). Extinction coefficient = 35,100 M-1 cm-1.

Licensing Information

Sold under license from Lucerna

Optical Data

Emission Color Green
λabs 485 nm
λem 527 nm
Extinction Coefficient (ε) 35100 M-1cm-1
Quantum Yield (φ) 0.64
Application Live-cell Imaging, Fluorescence Microscopy

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Product Specifications for TBI

Molecular Weight

386.38

Formula

C18H12F2N4O2S

Storage

Store at -20°C

Purity

≥98% (HPLC)

Chemical Name

(Z)-3-((1H-Benzo[d]imidazol-4-yl)methyl)-5-(3,5-difluoro-4-hydroxybenzylidene)-2-thioxoimidazolidin-4-one

CAS Number

2863639-29-4

InChI Key

FFVXYOZVQLWDIF-NSIKDUERSA-N

SMILES

FC1=C(C(F)=CC(/C=C2NC(N(C\2=O)CC3=C4N=CNC4=CC=C3)=S)=C1)O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 7.73 20

Preparing Stock Solutions for TBI

The following data is based on the product molecular weight 386.38.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.2 mM 12.94 mL 64.70 mL 129.41 mL
1 mM 2.59 mL 12.94 mL 25.88 mL
2 mM 1.29 mL 6.47 mL 12.94 mL
10 mM 0.26 mL 1.29 mL 2.59 mL

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Background References

References are publications that support the biological activity of the product.

Product Documents for TBI

Certificate of Analysis

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Product Specific Notices for TBI

For research use only

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