Recombinant Human TCEA1 His Protein

Novus Biologicals | Catalog # NBP1-98993

Novus Biologicals
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Key Product Details

Source

E. coli

Tag

His

Applications

SDS-PAGE
View all TCEA1 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-301 of Human TCEA1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMEDEVV RFAKKMDKMV QKKNAAGALD LLKELKNIPM TLELLQSTRI GMSVNAIRKQ STDEEVTSLA KSLIKSWKKL LDGPSTEKDL DEKKKEPAIT SQNSPEAREE STSSGNVSNR KDETNARDTY VSSFPRAPST SDSVRLKCRE MLAAALRTGD DYIAIGADEE ELGSQIEEAI YQEIRNTDMK YKNRVRSRIS NLKDAKNPNL RKNVLCGNIP PDLFARMTAE EMASDELKEM RKNLTKEAIR EHQMAKTGGT QTDLFTCGKC KKKNCTYTQV QTRSADEPMT TFVVCNECGN RWKFC

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

36.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human TCEA1 His Protein

SDS-PAGE: Recombinant Human TCEA1 His Protein [NBP1-98993]

SDS-PAGE: Recombinant Human TCEA1 His Protein [NBP1-98993]

SDS-Page: Recombinant Human TCEA1 Protein [NBP1-98993] - 15% SDS-PAGE (3ug)

Formulation, Preparation, and Storage

NBP1-98993
Formulation 20 mM Tris-HCl buffer (pH7.5), 20% glycerol, 1 mM DTT, 50 mM NaCl
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TCEA1

TCEA1 (Transcription elongation factor A protein 1) belongs to the TFS-II family. TCEA1 binds to Pol II and functions to cleave the nascent transcript, thereby unlocking the complex and allowing transcription to continue. Localized to the nucleus, TCEA1 contains three independently-folding domains, all of which are necessary for proper binding to Pol II. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus. Recombinant human TCEA1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

GTF2Stranscription elongation factor A protein 1, SII, TF2STCEA, TFIISTranscription elongation factor TFIIS.o, transcription elongation factor A (SII), 1, Transcription elongation factor S-II protein 1

Gene Symbol

TCEA1

Additional TCEA1 Products

Product Documents for Recombinant Human TCEA1 His Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Human TCEA1 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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