TCEB2 Antibody (23GB5610)

Novus Biologicals | Catalog # NBP3-51360

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB5610
View all TCEB2 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human TCEB2 (Uniprot: Q15370)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TCEB2 Antibody (23GB5610)

TCEB2 Antibody (23GB5610)

Flow Cytometry: TCEB2 Antibody (23GB5610)[NBP3-51360] -

Validation of TCEB2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with TCEB2 antibody (1:2,000) and analyzed using BD flow cytometer.
TCEB2 Antibody (23GB5610)

Flow Cytometry: TCEB2 Antibody (23GB5610)[NBP3-51360] -

Flow cytometric analysis of TCEB2expression in HepG2 cells using TCEB2 antibody (1:2,000). Green, isotype control; red, TCEB2.
TCEB2 Antibody (23GB5610)

Western Blot: TCEB2 Antibody (23GB5610)[NBP3-51360] -

Western blotting analysis using TCEB2 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for TCEB2 Antibody (23GB5610)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

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Advanced Features

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TCEB2

TCEB2 encodes the protein elongin B, which is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Alternate Names

18-kD subunit, ELOB, Elongin 18 kDa subunit, elongin-B, RNA polymerase II transcription factor SIII p18 subunit, RNA polymerase II transcription factor SIII subunit B, SIII p18, transcription elongation factor B (SIII), polypeptide 2 (18kD, elongin B), transcription elongation factor B (SIII), polypeptide 2 (18kDa, elongin B), transcription elongation factor B polypeptide 2

Gene Symbol

ELOB

Additional TCEB2 Products

Product Documents for TCEB2 Antibody (23GB5610)

Certificate of Analysis

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Product Specific Notices for TCEB2 Antibody (23GB5610)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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