TCP1 alpha Antibody (23c) - BSA Free

Novus Biologicals | Catalog # NB120-13501

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Chicken, Hamster, Plant, Rabbit, Sheep

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2C Clone # 23c

Format

BSA Free
View all TCP1 alpha Primary Antibodies »

Product Specifications

Immunogen

Recombinant mouse TCP-1 alpha (C-terminal fragment).

Epitope

Ab13501 recognises the COOH group, addition of an alanine (LDDA COOH) prevents binding in ELISA assays to immobilized synthetic peptide sequences.

Localization

Cytoplasmic

Specificity

This recognizes other proteins, most notably the p102B' COP subunit of Golgi coatomer. It does not react with human Hsp60 protein.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2C

Scientific Data Images for TCP1 alpha Antibody (23c) - BSA Free

Western Blot: TCP1 alpha Antibody (23c) [NB120-13501] Lane 1: MW marker, Lane 2: Hsp60 Recombinant Human Protein, Lane 3: 3T3 Heat Shocked, Lane 4: PC-12 Heat Shocked.

Applications for TCP1 alpha Antibody (23c) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

5 ug/ml

Immunoprecipitation

1:10-1:500

Western Blot

2-5 ug/ml
Application Notes
Detects TCP1 alpha in 20 ug of RK13 heat shocked cell lysate. Predicited molecular weight: 60 kDa. Also detects a molecular mass band of approximately 92 kDa.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS containing 50% glycerol.

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TCP1 alpha

T complex polypeptide 1 (TCP1) is a ~60 kDa protein constitutively expressed in almost all eukaryotic cells, and is upregulated during spermatogenesis. It is found in the cytosol as a subunit of a hetero-oligomeric chaperone that is known to be involved in the folding of actin and tubulin. The family of proteins termed chaperonins act to recognize and stabilize polypeptide intermediates during folding, assembly and disassembly, and share many characteristics with Heat Shock Protein 70 (HSP 70) including high abundance, induction by environmental stress, and ATPase activity. The chaperonin family includes the mitochondrial HSP60, Escherichia coli GroEL, the plastid Rubisco subunit binding protein, and the archaebacterial protein TF55. The TCP1 sequence shows nearly 40% identity to TF55, but only minimal similarity to HSP60 and GroEL.

Alternate Names

CCT1T-complex protein 1 subunit alpha, CCTA, CCT-alpha, D6S230E, tailless complex polypeptide 1, t-complex 1, T-complex protein 1, alpha subunit, TCP-1-alpha

Entrez Gene IDs

21454 (Mouse); 24818 (Rat)

Gene Symbol

TCP1

Additional TCP1 alpha Products

Product Documents for TCP1 alpha Antibody (23c) - BSA Free

Certificate of Analysis

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Product Specific Notices for TCP1 alpha Antibody (23c) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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