TCP1-beta Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-88147

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all TCP1-beta Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DALCVLAQTVKDSRTVYGGGCSEMLMAHAVTQLANRTPGKEAVAMESYAKALRMLPTIIADNAGYDSADLVAQLRAAHSEGNTTAGLDMREGTIGDMAILGITESFQVKRQVLLSAAEAAEVILRVDN

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TCP1-beta Antibody - BSA Free

Immunohistochemistry-Paraffin: TCP1-beta Antibody [NBP1-88147]

Immunohistochemistry-Paraffin: TCP1-beta Antibody [NBP1-88147]

Immunohistochemistry-Paraffin: TCP1-beta Antibody [NBP1-88147] - Staining of human cerebral cortex, skeletal muscle, testis and tonsil using Anti-CCT2 antibody NBP1-88147 (A) shows similar protein distribution across tissues to independent antibody NBP1-88146 (B).
Western Blot: TCP1-beta Antibody [NBP1-88147]

Western Blot: TCP1-beta Antibody [NBP1-88147]

Western Blot: TCP1-beta Antibody [NBP1-88147] - Staining of human tonsil.
Immunohistochemistry-Paraffin: TCP1-beta Antibody [NBP1-88147]

Immunohistochemistry-Paraffin: TCP1-beta Antibody [NBP1-88147]

Immunohistochemistry-Paraffin: TCP1-beta Antibody [NBP1-88147] - Staining of human skeletal muscle shows low expression as expected.
Immunohistochemistry-Paraffin: TCP1-beta Antibody [NBP1-88147]

Immunohistochemistry-Paraffin: TCP1-beta Antibody [NBP1-88147]

Immunohistochemistry-Paraffin: TCP1-beta Antibody [NBP1-88147] - Staining in human testis and skeletal muscle tissues using anti-CCT2 antibody. Corresponding CCT2 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: TCP1-beta Antibody [NBP1-88147]

Immunohistochemistry-Paraffin: TCP1-beta Antibody [NBP1-88147]

Immunohistochemistry-Paraffin: TCP1-beta Antibody [NBP1-88147] - Staining of human testis shows high expression.
TCP1-beta Antibody - BSA Free Immunohistochemistry-Paraffin: TCP1-beta Antibody - BSA Free [NBP1-88147]

Immunohistochemistry-Paraffin: TCP1-beta Antibody - BSA Free [NBP1-88147]

Staining of human cerebral cortex using Anti-CCT2 antibody HPA003197.
Western Blot: TCP1-beta Antibody [NBP1-88147]

Western Blot: TCP1-beta Antibody [NBP1-88147]

Western Blot: TCP1-beta Antibody [NBP1-88147] - Analysis using Anti-CCT2 antibody NBP1-88147 (A) shows similar pattern to independent antibody NBP1-88146 (B).
TCP1-beta Antibody - BSA Free Western Blot: TCP1-beta Antibody - BSA Free [NBP1-88147]

Western Blot: TCP1-beta Antibody - BSA Free [NBP1-88147]

Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)

Applications for TCP1-beta Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. IF fixation permeabilization: PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TCP1-beta

TCP1-beta encodes a molecular chaperone that is member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of the gene described in this record have been observed but have not been thoroughly characterized.

Alternate Names

Cctb, CCT-beta, CCTBPRO1633,99D8.1, chaperonin containing t-complex polypeptide 1, beta subunit, chaperonin containing t-complex polypeptide 1, subunit 2, chaperonin containing TCP1, subunit 2 (beta), MGC142074, MGC142076, T-complex protein 1 subunit beta, T-complex protein 1, beta subunit, TCP-1-beta

Gene Symbol

CCT2

Additional TCP1-beta Products

Product Documents for TCP1-beta Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for TCP1-beta Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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