TCP1-beta Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-38283

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all TCP1-beta Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 206-535 of human TCP1-beta (NP_006422.1).

Sequence:
GGSLADSYLDEGFLLDKKIGVNQPKRIENAKILIANTGMDTDKIKIFGSRVRVDSTAKVAEIEHAEKEKMKEKVERILKHGINCFINRQLIYNYPEQLFGAAGVMAIEHADFAGVERLALVTGGEIASTFDHPELVKLGSCKLIEEVMIGEDKLIHFSGVALGEACTIVLRGATQQILDEAERSLHDALCVLAQTVKDSRTVYGGGCSEMLMAHAVTQLANRTPGKEAVAMESYAKALRMLPTIIADNAGYDSADLVAQLRAAHSEGNTTAGLDMREGTIGDMAILGITESFQVKRQVLLSAAEAAEVILRVDNIIKAAPRKRVPDHHPC

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TCP1-beta Antibody - BSA Free

TCP1-beta Antibody

Immunocytochemistry/ Immunofluorescence: TCP1-beta Antibody [NBP3-38283] -

Immunocytochemistry/ Immunofluorescence: TCP1-beta Antibody [NBP3-38283] - Immunofluorescence analysis of HeLa cells using TCP1-beta Rabbit pAb. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
TCP1-beta Antibody

Western Blot: TCP1-beta Antibody [NBP3-38283] -

Western Blot: TCP1-beta Antibody [NBP3-38283] - Western blot analysis of various lysates using TCP1-beta Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.
TCP1-beta Antibody

Immunoprecipitation: TCP1-beta Antibody [NBP3-38283] -

Immunoprecipitation: TCP1-beta Antibody [NBP3-38283] - Immunoprecipitation analysis of 200 ug extracts of A-549 cells, using 3 ug TCP1-beta antibody. Western blot was performed from the immunoprecipitate using TCP1-beta antibody at a dilution of 1:1000.

Applications for TCP1-beta Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunoprecipitation

0.5ug - 4ug antibody for 200ug - 400ug extracts of whole cells

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TCP1-beta

TCP1-beta encodes a molecular chaperone that is member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of the gene described in this record have been observed but have not been thoroughly characterized.

Alternate Names

Cctb, CCT-beta, CCTBPRO1633,99D8.1, chaperonin containing t-complex polypeptide 1, beta subunit, chaperonin containing t-complex polypeptide 1, subunit 2, chaperonin containing TCP1, subunit 2 (beta), MGC142074, MGC142076, T-complex protein 1 subunit beta, T-complex protein 1, beta subunit, TCP-1-beta

Gene Symbol

CCT2

Additional TCP1-beta Products

Product Documents for TCP1-beta Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for TCP1-beta Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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