TCP1-delta Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-41360
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human, Mouse
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
The epitope is recognized to a region between residue 1 to 50 of human Chaperonin Containing Tcp1, Subunit 4 (Delta).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for TCP1-delta Antibody - BSA Free
Western Blot: TCP1-delta Antibody [NBP2-41360]
Western Blot: TCP1-delta Antibody [NBP2-41360] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CCT4 antibody NBP2-41360 used for IP at 6 ug per reaction. CCT4 was also immunoprecipitated by rabbit anti-CCT4 antibody BL18407. For blotting immunoprecipitated CCT4, it was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.Immunoprecipitation: TCP1-delta Antibody [NBP2-41360]
Immunoprecipitation: TCP1-delta Antibody [NBP2-41360] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CCT4 antibody NBP2-41360 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.Applications for TCP1-delta Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
2 - 10 ug/mg lysate
Western Blot
1:2000 - 1:10000
Application Notes
Validation by IP/Western Blot was performed using a 4-20% SDS-PAGE gel.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: TCP1-delta
Alternate Names
CCTD, CCT-DELTA, chaperonin containing t-complex polypeptide 1, delta subunit, chaperonin containing TCP1, subunit 4 (delta), MGC126165, SRBMGC126164, Stimulator of TAR RNA-binding, T-complex protein 1 subunit delta, TCP-1-delta
Gene Symbol
CCT4
UniProt
Additional TCP1-delta Products
Product Documents for TCP1-delta Antibody - BSA Free
Certificate of Analysis
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Product Specific Notices for TCP1-delta Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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