TFIIB Antibody (PCRP-GTF2B-1D1)

Novus Biologicals | Catalog # NBP3-13934

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunoprecipitation, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # PCRP-GTF2B-1D1
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Product Specifications

Immunogen

Recombinant full-length human TFIIB protein (Uniprot: Q00403)

Localization

Nucleus, Nucleoplasm, nuclear bodies

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

34.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14055)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for TFIIB Antibody (PCRP-GTF2B-1D1)

Flow Cytometry: TFIIB Antibody (PCRP-GTF2B-1D1) [NBP3-13934]

Flow Cytometry: TFIIB Antibody (PCRP-GTF2B-1D1) [NBP3-13934]

Flow Cytometry: TFIIB Antibody (PCRP-GTF2B-1D1) [NBP3-13934] - Flow cytometric analysis of PFA-fixed HeLa cells. TFIIB antibody (PCRP-GTF2B-1D1) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).
Protein Array: TFIIB Antibody (PCRP-GTF2B-1D1) [NBP3-13934]

Protein Array: TFIIB Antibody (PCRP-GTF2B-1D1) [NBP3-13934]

Protein Array: TFIIB Antibody (PCRP-GTF2B-1D1) [NBP3-13934] - Analysis of Protein Array containing more than 19,000 full-length human proteins using TFIIB Antibody (PCRP-GTF2B-1D1).

Applications for TFIIB Antibody (PCRP-GTF2B-1D1)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunoprecipitation

1-2 ug/100-500 ug protein
Application Notes
ELISA: For coating, order antibody without BSA

Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)

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Advanced Features

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Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: TFIIB

TFIIB encodes the general transcription factor IIB, one of the ubiquitous factors required for transcription initiation by RNA polymerase II. The protein localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. Transcription factor IIB serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II. [provided by RefSeq]

Alternate Names

general transcription factor IIB, General transcription factor TFIIB, S300-II, TFIIBTF2BRNA polymerase II transcription factor IIB, transcription initiation factor IIB

Gene Symbol

GTF2B

UniProt

Additional TFIIB Products

Product Documents for TFIIB Antibody (PCRP-GTF2B-1D1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for TFIIB Antibody (PCRP-GTF2B-1D1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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