TGF-beta 3 Magnetic Luminex® Performance Assay

Name: TGF-beta 3 Magnetic Luminex® Performance Assay
Brand: R&D Systems
SKU: LTGM300
Price: 210 USD
Availability: InStock

R&D Systems | Catalog # LTGM300

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Product Documents

Luminex High Performance Assays

TGF-beta 3 Magnetic Luminex® Performance Assay is specifically designed for multiplex detection of human TGF-beta 3 with a panel of related analytes in serum, plasma, or tissue culture supernatants. Luminex High Performance Assays go through rigorous development, validation, and quality control testing to ensure the best performance of your multiplex assay.

This assay is customizable, select some or all the analytes in the entire panel. Customize your panel with the Luminex Assay Customization Tool.

Build Now

Build Your Assay
Product Features
Performance Validation
Kit Contents
Precision and Accuracy
Which Luminex Assay is Right for Me
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Panel

Human TGF-beta Panel

Assay Type

Magnetic bead-based multiplex assay for the Luminex® platform

Format

1 x 96-well microplate and magnetic antibody-coated beads

Species

Human

Analytes Detected

TGF-beta 3

Not finding your analyte of interest? View our complete Luminex offering in the full version of our Luminex Assay Customization Tool. We also offer Custom Luminex Services.

View all TGF-beta 3 Luminex Assays »

Build Your Assay

Make Selections Below to Configure Your Custom Luminex® Assay

Configured Assays

Below are kits that are available based on your selections. These selections may be further customized before adding to the cart or emailing the configuration to your purchasing department or local distributor to place your order. Selected analytes may be included in more than one High Performance Assay. All assay options are displayed.

If overlapping bead regions occur in analyte selections, send your Luminex configuration code to our team for resolution.

How would you like these analytes mixed?

Premixed

User Mixed

Please select at least one mixing option to see the kits.

Would you like to split based on sample dilution?

Do not split

Split based on serum

Split based on plasma

Split based on cell culture supernates

Product Features

Magnetic Luminex High Performance Assays are flexible bead-based multiplex assays. They allow up to 50 user-defined target analytes to be simultaneously profiled using cell culture supernates, serum, or plasma samples. Some panels are also validated with human milk, saliva, and/or urine samples.

Simultaneously profile Human TGF-beta 3 with a panel of related analytes in one sample
Magnetic format allows for easier wash steps
Undergoes similar validation testing as Quantikine® ELISA Kits
Precise and Specific
Requires a small sample volume (<50 µL)
Run your assay in just 3-5 hours
Mass-calibrated standards for consistent results with every new lot of material
Enough reagents for one 96-well plate
Quick turn-around time: Kits are typically shipped within 2-3 days of ordering*

*Please allow additional time for international orders.

Performance Validation

R&D Systems Luminex High Performance Assays undergo our most extensive validation testing. All Luminex High Performance Assay Panels are validated for use with serum and plasma samples. Some High Performance Assays are additionally validated for cell culture supernatants, milk, saliva, or urine, as indicated on the individual product data sheets. Luminex High Performance Assays are tested for sensitivity (three-quarters the low standard), intra-assay precision, inter-assay precision, and to ensure assay linearity for validated sample types. Recovery values for individual samples in validated sample types are also tested. Validation data for each analyte can be found in the product datasheet.

Base Kit Contents

Standard Cocktail(s)
Bead Diluent
Biotin Antibody Diluent
Calibrator Diluent
Wash Buffer
Streptavidin-PE
One flat-bottom 96-well Microplate
Foil Plate Sealers (4)
Mixing Bottle
Standard Value Card**

**A standard curve must be generated each time an assay is run, utilizing values from the Certificate of Analysis provided with each Luminex Assay.

Bead Set Contents

User-mixed antibody-coated Magnetic beads in individual vials
User-mixed cocktail of biotinylated detection antibody

Precision

	Intra-Assay Precision			Inter-Assay Precision
Intra-assay Precision (precision within an assay) - Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (precision between assays) - Three samples of known concentration were tested in ninety-four separate assays to assess precision between assays.
Sample	1	2	3	1	2	3
n	20	20	20	94	94	94
Mean (pg/mL)	174	1499	11,266	158	1498	10,672
Standard deviation	17	137	672	27	186	1208
CV (%)	9.8	9.1	6.0	17.1	12.4	11.3

Recovery

Sample Type	Average % Recovery	Range (%)
Samples containing and/or spiked with human TGF-B1 were evaluated for recovery.
Cell culture supernate	96	65-124
Serum	76	65-92
EDTA plasma	82	73-97
Heparin plasma	83	72-98
Platelet-poor EDTA plasma	89	69-110
Platelet-poor heparin plasma	88	64-115
Urine	106	86-125

Linearity

		Cell culture supernate	Serum	EDTA plasma	Heparin plasma	Platelet-poor EDTA plasma	Platelet-poor Heparin plasma	Urine
Samples containing and/or spiked with human TGF-B1 were serially diluted to evaluate assay linearity.
1:2	Average % of Expected	98	103	100	100	106	108	93
1:2	Range (%)	83-120	96-113	81-111	90-112	94-120	90-123	82-109
1:4	Average % of Expected	91	108	101	102	103	103	83
1:4	Range (%)	75-120	97-122	83-114	91-119	83-127	84-125	69-99
1:8	Average % of Expected	88	109	101	101	104	103	80
1:8	Range (%)	72-114	96-119	83-111	86-111	82-129	80-130	66-107

Which Luminex Assay is Right for Me?

Assays for the Luminex platform are offered as High Performance Assays or Discovery Assays.

High Performance Assays are optimized and validated as complete panels with a focused set of analytes. You can choose from Fixed Panels, Premixed options, or Configurable User Mix formats depending on your needs.

Discovery Assays deliver maximum flexibility, enabling the creation of fully customized panels tailored to your research needs. With universal reagents validated for compatibility across all analytes in the menu, they support endless customization possibilities.

Additional Information About Luminex Assays

All R&D Systems Luminex Assays are designed for use with any Luminex instrument including MAGPIX, Luminex 200, FLEXMAP 3D, and the INTELLIFLEX.

Luminex assays use color-coded, magnetic microparticles coated with analyte-specific antibodies to enable multiplex quantitation. During the assay, standards and samples are added to the wells, where target analytes bind to the capture antibodies. A biotinylated detection antibody cocktail is then introduced, followed by a Streptavidin-PE conjugate that generates a fluorescent signal proportional to the amount of analyte present.

Each microparticle is identified by a unique signature and the signal intensity is measured using a Luminex instrument. These assays are designed to deliver reliable and reproducible results with validated antibody pairs and optimized protocols. They are well suited for applications that require sensitivity, accuracy, and confidence in your data while enabling efficient multiplexing to measure multiple targets simultaneously.

Scientific Data Images for TGF-beta 3 Magnetic Luminex® Performance Assay

Quantist software graph of signal intensity versus concentration for TGF-beta 3 in the Human TGF-beta Luminex Panel

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TGF-beta 3

TGF-beta 3 (transforming growth factor beta 3) Is a pleiotropic cytokine that regulates immune function, proliferation, and epithelial-mesenchymal transition. Disulfide-linked homodimers of LAP and TGF-beta 3 remain non-covalently associated after secretion, forming the small latent TGF-beta 3 complex. Covalent linkage of LAP to one of three latent TGF-beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix. Bioactive TGF-beta is released by proteolytic cleavage. TGF-beta 3 signals through a receptor complex containing TGF-beta RII and either TGF-beta RI/ALK5 or ALK1.