Sample Activation Kit 1

For acid activation and neutralization of samples in various immunoassays
Catalog # Availability Size / Price Qty
DY010
R&D Systems DuoSet Ancillary Reagent Kits
1 Image
Product Details
Citations (5)
FAQs
Supplemental Products
Reviews (4)

Sample Activation Kit 1 Summary

INTENDED USE & DESCRIPTION

Sample Activation Kit 1 (Part # 993078) offers convenient, premixed reagents for the activation of samples containing TGF-beta. The kit contains 3 vials of 1N HCL (Part # 895094, 10 mL/vial) and 3 vials of 1.2N NaOH/0.5M HEPES (Part # 895096, 10 mL/vial) for activation of samples in all of R&D Systems’ TGF-beta related products, including DuoSet® ELISA Development Systems and Quantikine® ELISAs, the Luminex® Performance Assays.

STORAGE & STABILITY

Store unopened at room temperature. Do not use past kit expiration. Stable for up to 30 days at room temperature after opening provided this is within the expiration date of the kit. Contains no preservatives.

PROCEDURE & EXPECTED VALUES

Follow activation procedure provided with each application. The kit contains reagents in quantities sufficient for 5 ELISA plates worth of samples. 

TECHNICAL HINTS & LIMITATIONS OF THE PROCEDURE 

  • Caution: May cause skin, eye, and respiratory irritation. Avoid breathing fumes. Wear protective gloves, clothing, and eye and face protection. Wash hands thoroughly after handling. Please refer to the MSDS on our website prior to use. 

Specifications

Shipping Conditions
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at room temperature. Do not use past expiration date.
Applications
ELISA
Species
Multi-Species

Product Datasheets

Citations for Sample Activation Kit 1

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Palladin isoforms 3 and 4 regulate cancer-associated fibroblast pro-tumor functions in pancreatic ductal adenocarcinoma
    Authors: JI Alexander, DB Vendramini, R Francescon, T Luong, J Franco-Bar, N Shah, JC Gardiner, E Nicolas, KS Raghavan, E Cukierman
    Scientific Reports, 2021;11(1):3802.  2021
  2. A-Kinase Anchoring Proteins Diminish TGF-&beta1/Cigarette Smoke-Induced Epithelial-To-Mesenchymal Transition
    Authors: H Zuo, M Trombetta-, IH Heijink, CHTJ van der Ve, L Hesse, KN Faber, WJ Poppinga, H Maarsingh, VO Nikolaev, AM Schmidt
    Cells, 2020;9(2):.  2020
  3. Inhibition of Myostatin Reduces Collagen Deposition in a Mouse Model of Oculopharyngeal Muscular Dystrophy (OPMD) With Established Disease
    Authors: P Harish, L Forrest, S Herath, G Dickson, A Malerba, L Popplewell
    Front Physiol, 2020;11(0):184.  2020
  4. Age-induced accumulation of methylmalonic acid promotes tumour progression
    Authors: AP Gomes, D Ilter, V Low, JE Endress, J Fernández-, A Rosenzweig, T Schild, D Broekaert, A Ahmed, M Planque, I Elia, J Han, C Kinzig, E Mullarky, AP Mutvei, J Asara, R de Cabo, LC Cantley, N Dephoure, SM Fendt, J Blenis
    Nature, 2020;0(0):.  2020
  5. Myoferlin regulates epithelial cancer cell plasticity and migration through autocrine TGF-?1 signaling
    Authors: VR Barnhouse, JL Weist, VC Shukla, SN Ghadiali, DA Kniss, JL Leight
    Oncotarget, 2018;9(27):19209-19222.  2018

FAQs

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Reviews for Sample Activation Kit 1

Average Rating: 5 (Based on 4 Reviews)

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Sample Activation Kit 1
By Anonymous on 04/09/2021
Application:

Used this with DY243 and the ancillary kit. Very recommended


Sample Activation Kit 1
By Anonymous on 10/10/2020
Application:

We use this in our lab along with the mouse TGF-beta ELISA kit. Activates the samples, straightforward to use, will yield enough for multiple plates.


Sample Activation Kit 1
By Anonymous on 08/23/2019
Application:

Sample Activation Kit 1
By Anonymous on 03/28/2019
Application:

20uL of rat plasma was acid activated with 10uL of 1N HCl for 10 mins at RT. Samples were neutralized with 10uL of 1.2N NaOH. non-acid treated plasma was tested at a 1:10 dilution and acid treated samples at 1:20.