Sample Activation Kit 1

For acid activation and neutralization of samples in various immunoassays
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R&D Systems DuoSet Ancillary Reagent Kits
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Product Details
Citations (10)
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Reviews (5)

Sample Activation Kit 1 Summary


Sample Activation Kit 1 (Part # 993078) offers convenient, premixed reagents for the activation of samples containing TGF-beta. The kit contains 3 vials of 1N HCL (Part # 895094, 10 mL/vial) and 3 vials of 1.2N NaOH/0.5M HEPES (Part # 895096, 10 mL/vial) for activation of samples in all of R&D Systems’ TGF-beta related products, including DuoSet® ELISA Development Systems and Quantikine® ELISAs, the Luminex® Performance Assays.


Store unopened at room temperature. Do not use past kit expiration. Stable for up to 30 days at room temperature after opening provided this is within the expiration date of the kit. Contains no preservatives.


Follow activation procedure provided with each application. The kit contains reagents in quantities sufficient for 5 ELISA plates worth of samples. 


  • Caution: May cause skin, eye, and respiratory irritation. Avoid breathing fumes. Wear protective gloves, clothing, and eye and face protection. Wash hands thoroughly after handling. Please refer to the MSDS on our website prior to use. 


Shipping Conditions
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Store the unopened product at room temperature. Do not use past expiration date.

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Citations for Sample Activation Kit 1

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Role of Betaglycan in TGF-beta Signaling and Wound Healing in Human Endometriotic Epithelial Cells and in Endometriosis
    Authors: AN Mwaura, MA Riaz, JB Maoga, E Mecha, COA Omwandho, G Scheiner-B, I Meinhold-H, L Konrad
    Biology, 2022;11(4):.  2022-01-01
  2. Fatty acid nitroalkene reversal of established lung fibrosis
    Authors: A Koudelka, V Cechova, M Rojas, N Mitash, A Bondonese, C St Croix, MA Ross, BA Freeman
    Redox Biology, 2022;50(0):102226.  2022-01-01
  3. A TGFbetaR inhibitor represses keratin-7 expression in 3D cultures of human salivary gland progenitor cells
    Authors: EW Fowler, EJ van Venroo, RL Witt, X Jia
    Scientific Reports, 2022;12(1):15008.  2022-01-01
  4. Peripheral blood CD4posCD25posFoxP3pos cells and inflammatory cytokines as biomarkers of response in rheumatoid arthritis patients treated with CTLA4-Ig
    Authors: G Elisa, B Tolusso, L Petricca, C Di Mario, MR Gigante, G Ferracciol, S Alivernini
    Arthritis Research & Therapy, 2022;24(1):143.  2022-01-01
  5. Exposure modality influences viral kinetics but not respiratory outcome of COVID-19 in multiple nonhuman primate species
    Authors: AC Fears, BJ Beddingfie, NR Chirichell, N Slisarenko, SZ Killeen, RK Redmann, K Goff, S Spencer, B Picou, N Golden, CC Midkiff, DJ Bush, LM Branco, ML Boisen, H Gao, DC Montefiori, RV Blair, LA Doyle-Meye, K Russell-Lo, NJ Maness, CJ Roy
    PloS Pathogens, 2022;18(7):e1010618.  2022-01-01
  6. Palladin isoforms 3 and 4 regulate cancer-associated fibroblast pro-tumor functions in pancreatic ductal adenocarcinoma
    Authors: JI Alexander, DB Vendramini, R Francescon, T Luong, J Franco-Bar, N Shah, JC Gardiner, E Nicolas, KS Raghavan, E Cukierman
    Scientific Reports, 2021;11(1):3802.  2021-01-01
  7. Inhibition of Myostatin Reduces Collagen Deposition in a Mouse Model of Oculopharyngeal Muscular Dystrophy (OPMD) With Established Disease
    Authors: P Harish, L Forrest, S Herath, G Dickson, A Malerba, L Popplewell
    Front Physiol, 2020;11(0):184.  2020-01-01
  8. A-Kinase Anchoring Proteins Diminish TGF-&beta1/Cigarette Smoke-Induced Epithelial-To-Mesenchymal Transition
    Authors: H Zuo, M Trombetta-, IH Heijink, CHTJ van der Ve, L Hesse, KN Faber, WJ Poppinga, H Maarsingh, VO Nikolaev, AM Schmidt
    Cells, 2020;9(2):.  2020-01-01
  9. Age-induced accumulation of methylmalonic acid promotes tumour progression
    Authors: AP Gomes, D Ilter, V Low, JE Endress, J Fernández-, A Rosenzweig, T Schild, D Broekaert, A Ahmed, M Planque, I Elia, J Han, C Kinzig, E Mullarky, AP Mutvei, J Asara, R de Cabo, LC Cantley, N Dephoure, SM Fendt, J Blenis
    Nature, 2020;0(0):.  2020-01-01
  10. Myoferlin regulates epithelial cancer cell plasticity and migration through autocrine TGF-?1 signaling
    Authors: VR Barnhouse, JL Weist, VC Shukla, SN Ghadiali, DA Kniss, JL Leight
    Oncotarget, 2018;9(27):19209-19222.  2018-01-01


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DuoSet ELISA Development Systems

Luminex High Performance Human TGF-beta Magnetic Panel

Parameter Colorimetric ELISA / Assay Kits

Quantikine Colorimetric Sandwich ELISAs

Reviews for Sample Activation Kit 1

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Sample Activation Kit 1
By Anonymous on 03/03/2023

Very stable and have long shelf life

Sample Activation Kit 1
By Anonymous on 04/09/2021

Used this with DY243 and the ancillary kit. Very recommended

Sample Activation Kit 1
By Cristina Caldari on 10/10/2020

We use this in our lab along with the mouse TGF-beta ELISA kit. Activates the samples, straightforward to use, will yield enough for multiple plates.

Sample Activation Kit 1
By Anonymous on 08/23/2019

Sample Activation Kit 1
By Anonymous on 03/28/2019

20uL of rat plasma was acid activated with 10uL of 1N HCl for 10 mins at RT. Samples were neutralized with 10uL of 1.2N NaOH. non-acid treated plasma was tested at a 1:10 dilution and acid treated samples at 1:20.